Rat central amygdala corticotropin-releasing hormone-expressing neurons projects to BST (Bed nuclei of the stria terminalis)

Cre-dependent AAV5-EF1a-DIO-NpHR-eYFP injected into the CeA of Crh-Cre rats produced eYFP-labelled CRF axon terminals in the ventral BNST, where optical fibers were placed; histology confirmed viral expression at the CeA injection site and in the BNST target.

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) is unsure. “The projection is implied by terminal photoinhibition, but the cited figure caption only mentions images of the injection and illumination sites, not explicit anterograde eYFP terminal labeling in BNST.”