Sources of Synaptic Input to Neurons in the Nucleus Accumbens Shell That Project to the Ventral Pallidum

PVT (Paraventricular nucleus of the thalamus) UBERON:0001920 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

Rabies input cells were consistently found in the anterior PVT, which supplied about a tenth of inputs and was a stronger source than several other areas; the posterior thalamus lacked input cells.

Fig. 3a,b; Figs. 4d-e, 5c

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ILA (Infralimbic area) UBERON:8440033 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

Infralimbic cortex contained quantified input cells; prefrontal cortex was a relatively weak source overall.

Fig. 3a,b

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LPO (Lateral preoptic area) UBERON:0001931 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

The lateral preoptic area contained input cells, described as a moderately strong source.

Fig. 3a,b; Figs. 4c, 5b

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DP (Dorsal peduncular area) UBERON:8440040 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

Dorsal peduncular cortex was among the quantified regions with at least 50 rabies input cells; prefrontal inputs were relatively weak.

Fig. 3a,b

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BST (Bed nuclei of the stria terminalis) UBERON:0001880 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

Rabies input cells were found in the bed nucleus of the stria terminalis, a moderately strong source.

Fig. 3a,b; Fig. 4b-c

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PPN (Pedunculopontine nucleus) UBERON:0002142 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

The pedunculopontine tegmental nucleus was a quantified source of rabies input cells.

Fig. 3a,b

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PL (Prelimbic area) UBERON:8440032 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

Prelimbic cortex contained quantified input cells; prefrontal inputs were relatively weak.

Fig. 3a,b

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CLA (Claustrum) UBERON:0002023 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

The claustrum was among regions quantified as containing rabies input cells to the shell-VP neurons.

Fig. 3a,b

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PRT (Pretectal region) UBERON:0001944 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

The precommissural nucleus contained quantified rabies input cells.

Fig. 3a,b

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DMH (Dorsomedial nucleus of the hypothalamus) UBERON:0001934 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

Input cells were quantified in the dorsomedial hypothalamus, a moderately strong source to the shell-VP neurons.

Fig. 3a,b

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SUB (Subiculum) UBERON:0002191 projects to LS (Lateral septal nucleus) UBERON:0002667 · Rat

Dense GFP fibers from accumbens-projecting vSub neurons were seen in the ventral and intermediate lateral septum just above the septal pole of the accumbens.

Fig. 6c-f

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SUB (Subiculum) UBERON:0002191 projects to ACB (Nucleus accumbens) UBERON:0001882 · Rat

Intersectional GFP labelling of vSub neurons projecting to the accumbens produced a dense fiber plexus in the dorsomedial shell, with fibers also in the immediately adjacent core.

Fig. 6c-f

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SUB (Subiculum) UBERON:0002191 projects to PALv (Pallidum, ventral region) UBERON:0002778 · Rat

Labelled fibers from accumbens-projecting vSub neurons largely avoided the VP lying between the olfactory tubercle and the shell.

Fig. 6; Results, Intersectional Anterograde Tracing

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PT (Parataenial nucleus) UBERON:0002992 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

The paratenial thalamic nucleus consistently contained input cells, a moderately strong source though weaker than aPVT.

Fig. 3a,b; Figs. 4d-e, 5c

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

LS (Lateral septal nucleus) UBERON:0002667 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

Input cells were scattered in the septum, mostly in the intermediate lateral septal nucleus, a moderately strong input source.

Fig. 3a,b; Figs. 4c, 5a

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

VMH (Ventromedial hypothalamic nucleus) UBERON:0001935 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

The ventromedial hypothalamus was among regions quantified for rabies input cells.

Fig. 3a,b

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PALv (Pallidum, ventral region) UBERON:0002778 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

Ventral pallidum neurons were rabies-labelled as inputs, contributing roughly a tenth of the input cells to the shell-VP projection neurons.

Fig. 3a,b; Figs. 4b, 5b

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

LHA (Lateral hypothalamic area) UBERON:0002430 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

Input cells were quantified in the peduncular lateral hypothalamus.

Fig. 3a,b; Fig. 4

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CA1 (Field CA1) UBERON:0003881 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

Field CA1 of the hippocampus contained rabies-labelled input cells to the accumbens shell neurons projecting to VP, described as a moderately strong source.

Fig. 3a,b; Fig. 4g

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ACB (Nucleus accumbens) UBERON:0001882 projects to PALv (Pallidum, ventral region) UBERON:0002778 · Rat

Retrograde AAV-Cre injected into ventromedial VP, combined with Cre-dependent helper and rabies in the medial shell, yielded hundreds of starter cells in the dorsomedial shell, identifying shell neurons projecting to VP.

Fig. 2

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VTA (Ventral tegmental area) UBERON:0002691 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

A few input cells were consistently seen in the VTA in every section examined.

Fig. 3a,b; Figs. 4f, 5d

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Isocortex UBERON:0001950 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

Medial orbital cortex was a quantified source of rabies input cells, part of the relatively weak prefrontal input.

Fig. 3a,b

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CEA (central amygdaloid nucleus) UBERON:0002883 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

Amygdalar input cells were concentrated in part in the central nucleus; the amygdala overall was a relatively weak source.

Fig. 3a,b; Fig. 4e

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SUB (Subiculum) UBERON:0002191 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

With intersectional monosynaptic rabies tracing from medial shell neurons projecting to ventromedial VP, the ventral subiculum held the largest number of input cells, more than 30% of all inputs, significantly more than other areas.

Fig. 3a,b; Figs. 4g, 5e-f

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

SUB (Subiculum) UBERON:0002191 projects to OT (Olfactory tubercle) UBERON:0001883 · Rat

More weakly labelled vSub fibers were present in the olfactory tubercle.

Fig. 6; Results, Intersectional Anterograde Tracing

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (basal amygdaloid nucleus) UBERON:0002887 synapses onto ACB (Nucleus accumbens) UBERON:0001882 · Rat

A scattering of input cells occurred in the basolateral amygdala, characterized as a relatively weak input.

Fig. 3a,b; Fig. 4e

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.