Low-threshold spiking interneurons perform feedback inhibition in the lateral amygdala

LA glutamatergic principal neurons nt-659aayyts5 functionally connects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

All three BL-responsive LA SOM+ interneurons tested by paired recording received input from a presynaptic LA principal cell.

Fig. 8c, d; Results

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

LA glutamatergic principal neurons nt-659aayyts5 functionally connects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

For seven fast-spiking or stuttering interneurons, none of the 32 neighbouring principal neurons tested evoked unitary EPSPs.

Fig. 5a; Results, interneuron-principal neuron pairs

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 functionally connects to LA glutamatergic principal neurons nt-659aayyts5 · Mouse

Electrical stimulation of the BL in slices evoked fixed-latency antidromic spikes in about a fifth of LA principal neurons, plus variable-latency polysynaptic IPSCs that were largely abolished by glutamate receptor blockade, consistent with recurrent recruitment of local LA interneurons.

Fig. 1a3, Fig. 1b; Results, 'BL Stimulation and inhibition in LA neurons'

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) disagrees. “The direction is reversed: the spikes were antidromic in LA axons projecting to BL, and the paper states LA-to-BL transfer is largely unidirectional, so BL is not shown to input LA principal cells.”

LA glutamatergic principal neurons nt-659aayyts5 functionally connects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

Paired recordings showed that LA principal neurons gave small, facilitating unitary EPSPs to LTS feedback interneurons; 13 of 35 principal cells tested were connected.

Fig. 4b, Fig. 5b; Results, interneuron-principal neuron pairs

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 functionally connects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

BL stimulation at 100 µA made a subset of LA SOM-tdTomato interneurons fire; these were the cells with the lowest spike thresholds.

Fig. 8a, b; Results, SOM- and PV-cre tomato section

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) disagrees. “The paper uses BL stimulation to antidromically recruit LA principal neurons, so it does not establish BL as the source of input to LA SOM+ cells.”

BLA (Basolateral amygdalar nucleus) MBA:295 functionally connects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

Only one of nine LA PV-tdTomato interneurons spiked in response to BL stimulation. At higher intensities the PV cells showed only small responses.

Fig. 8a, b; Results

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) disagrees. “The 1/9 PV spiking reflects antidromic recruitment of LA recurrent circuitry in the paper's interpretation, not a BL projection to LA PV cells.”

LA (Lateral amygdalar nucleus) MBA:131 functionally connects to LA glutamatergic principal neurons nt-659aayyts5 · Mouse

Spiking LTS feedback interneurons evoked unitary IPSPs in LA principal neurons. Their slow rise kinetics matched those of the polysynaptic IPSPs seen between principal cells.

Fig. 4b, Fig. 5b, d; Results

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

LA (Lateral amygdalar nucleus) MBA:131 functionally connects to LA glutamatergic principal neurons nt-659aayyts5 · Mouse

In each of the three BL-responsive SOM+ interneurons tested, the SOM+ cell in turn innervated its presynaptic principal cell, so the pairs were reciprocally connected.

Fig. 8c, d; Results

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

LA (Lateral amygdalar nucleus) MBA:131 functionally connects to LA glutamatergic principal neurons nt-659aayyts5 · Mouse

Fast-spiking non-feedback interneurons evoked unitary IPSPs with fast rise times in most of the LA principal neurons tested.

Fig. 5a, d; Results

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.