Genetic labeling of the nucleus of tractus solitarius neurons associated with electrical stimulation of the cervical or auricular vagus nerve in mice

NTS (Nucleus of the solitary tract) MBA:651 projects to DMH (Dorsomedial nucleus of the hypothalamus) MBA:830 · Mouse

The dorsomedial hypothalamic nucleus had relatively weaker mCherry terminal labeling from cNTS neurons than other hypothalamic targets.

Fig. 9D, D1

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NTS (Nucleus of the solitary tract) MBA:651 projects to SPVC (Spinal nucleus of the trigeminal, caudal part) MBA:429 · Mouse

Labeled cNTS terminals were found in the caudal spinal trigeminal nucleus, more weakly than in the lateral parabrachial nucleus.

Fig. 7B

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NTS (Nucleus of the solitary tract) MBA:651 projects to MD (Mediodorsal nucleus of thalamus) MBA:362 · Mouse

TRAPped cNTS axon terminals were observed in the mediodorsal thalamus, including its lateral, central and medial parts.

Fig. 9A–B, A1–B1

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NTS (Nucleus of the solitary tract) MBA:651 projects to PVT (Paraventricular nucleus of the thalamus) MBA:149 · Mouse

mCherry-labeled cNTS terminals were found in the midline PV thalamic nucleus. Terminal density did not differ between cVNS and aVNS mice.

Fig. 9B, B1, E

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NTS (Nucleus of the solitary tract) MBA:651 projects to MDRN (Medullary reticular nucleus) MBA:395 · Mouse

Anterogradely labeled terminals from VNS-TRAPped cNTS neurons were present in the medullary reticular formation, at relatively weak density.

Fig. 7B

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NTS (Nucleus of the solitary tract) MBA:651 projects to VMH (Ventromedial hypothalamic nucleus) MBA:693 · Mouse

mCherry-labeled terminals from VNS-TRAPped cNTS neurons were found in the ventromedial hypothalamic nucleus.

Fig. 9D, D1

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NTS (Nucleus of the solitary tract) MBA:651 projects to MRN (Midbrain reticular nucleus) MBA:128 · Mouse

Many mCherry-labeled terminals from VNS-TRAPped cNTS neurons were observed in the midbrain reticular formation.

Fig. 8; Results, PAG section

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NTS (Nucleus of the solitary tract) MBA:651 projects to PB (Parabrachial nucleus) MBA:867 · Mouse

Cre-dependent AAV-mCherry in VNS-TRAPped cNTS neurons labeled axon terminals bilaterally in the parabrachial nucleus. The lateral parabrachial nucleus was the most densely labeled hindbrain target, and the medial and externolateral parts had weaker labeling. Density was similar after cVNS and aVNS.

Fig. 7C–E, G

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NTS (Nucleus of the solitary tract) MBA:651 projects to AI (Agranular insular area) MBA:95 · Mouse

After cVNS, scattered and weak mCherry terminal labeling appeared in the insular cortex, mainly its agranular part. No cortical terminals were seen after aVNS.

Results, forebrain section (data not shown)

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NTS (Nucleus of the solitary tract) MBA:651 projects to CEAc (Central amygdalar nucleus, capsular part) MBA:544 · Mouse

No labeled cNTS terminals were observed in the CeC subdivision of the central amygdala in either cVNS or aVNS mice.

Fig. 9C, C1

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NTS (Nucleus of the solitary tract) MBA:651 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

The basolateral amygdala contained no mCherry-labeled terminals from TRAPped cNTS neurons after either stimulation type.

Fig. 9C, C1

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NTS (Nucleus of the solitary tract) MBA:651 projects to VPMpc (Ventral posteromedial nucleus of the thalamus, parvicellular part) MBA:741 · Mouse

In aVNS-TRAPped mice, condensed mCherry terminal labeling was seen in the parvicellular part of the VPM.

Fig. 9A1–B1

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Xn (vagus nerve) MBA:917 functionally connects to NTS (Nucleus of the solitary tract) MBA:651 · Mouse

Electrically stimulating the left cervical vagus trunk or the auricular vagal territory of the ear induced strong c-fos expression and Fos-TRAP mCherry labeling in caudal NTS neurons on both sides, with more on the ipsilateral side. Sham animals showed rare or no labeling.

Fig. 2; Fig. 3–5

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NTS (Nucleus of the solitary tract) MBA:651 projects to LC (Locus ceruleus) MBA:147 · Mouse

TRAPped cNTS axon terminals were observed in the locus coeruleus, at relatively weak density.

Fig. 7D

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NTS (Nucleus of the solitary tract) MBA:651 projects to DR (Dorsal nucleus raphe) MBA:872 · Mouse

A considerable number of labeled cNTS terminals were found in the dorsal and lateral subdivisions of the dorsal raphe after both cVNS and aVNS.

Fig. 8A–B

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NTS (Nucleus of the solitary tract) MBA:651 projects to VPM (Ventral posteromedial nucleus of the thalamus) MBA:733 · Mouse

Labeled cNTS terminals were observed in the thalamic VPM after cVNS and aVNS. Labeling was more condensed after aVNS.

Fig. 9A–B, A1–B1

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NTS (Nucleus of the solitary tract) MBA:651 projects to TU (Tuberal nucleus) MBA:614 · Mouse

TRAPped cNTS terminals were observed in the medial tuberal nucleus of the hypothalamus in both stimulation groups.

Fig. 9D, D1

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NTS (Nucleus of the solitary tract) MBA:651 projects to CEAm (Central amygdalar nucleus, medial part) MBA:559 · Mouse

Within the central amygdala, terminals from VNS-TRAPped cNTS neurons were dense in the medial subdivision. CeA terminal density was similar between cVNS and aVNS.

Fig. 9C, C1, F

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NTS (Nucleus of the solitary tract) MBA:651 projects to PCN (Paracentral nucleus) MBA:907 · Mouse

cNTS terminals labeled with mCherry were found in the paracentral thalamic nucleus, with condensed labeling in aVNS mice.

Fig. 9A–B, A1–B1

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NTS (Nucleus of the solitary tract) MBA:651 projects to MY (Medulla) MBA:354 · Mouse

mCherry-labeled terminals from TRAPped cNTS neurons were present in the rostral ventromedial medulla, with similar density after cervical and auricular VNS.

Fig. 7A, F

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NTS (Nucleus of the solitary tract) MBA:651 projects to VPL (Ventral posterolateral nucleus of the thalamus) MBA:718 · Mouse

TRAPped cNTS terminals were present in the VPL of the thalamus after both forms of vagal stimulation.

Fig. 9A–B, A1–B1

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NTS (Nucleus of the solitary tract) MBA:651 projects to PAG (Periaqueductal gray) MBA:795 · Mouse

Many labeled cNTS terminals were found in the PAG, mostly in its lateral and ventral subdivisions rather than the dorsal part. Terminal density in the dorsolateral PAG was higher after cVNS than after aVNS.

Fig. 8A–D

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NTS (Nucleus of the solitary tract) MBA:651 projects to PARN (Parvicellular reticular nucleus) MBA:852 · Mouse

TRAPped cNTS axon terminals were observed in the parvicellular reticular formation. Labeling there was weaker than in the lateral parabrachial nucleus.

Fig. 7A

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NTS (Nucleus of the solitary tract) MBA:651 projects to CEAl (Central amygdalar nucleus, lateral part) MBA:551 · Mouse

The lateral subdivision of the central amygdala had relatively weaker mCherry terminal labeling from cNTS neurons than the medial subdivision.

Fig. 9C, C1

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NTS (Nucleus of the solitary tract) MBA:651 projects to LHA (Lateral hypothalamic area) MBA:194 · Mouse

Labeled cNTS axon terminals were observed in the lateral hypothalamus, with similar density after cVNS and aVNS.

Fig. 9D, D1, G

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NTS (Nucleus of the solitary tract) MBA:651 projects to CM (Central medial nucleus of the thalamus) MBA:599 · Mouse

Labeled terminals from VNS-TRAPped cNTS neurons were found in the centromedial thalamic nucleus.

Fig. 9A–B, A1–B1

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NTS (Nucleus of the solitary tract) MBA:651 projects to KF (Koelliker-Fuse subnucleus) MBA:123 · Mouse

mCherry-labeled cNTS terminals were found in the Kölliker-Fuse nucleus, more weakly than in the lateral parabrachial nucleus.

Fig. 7C

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NTS (Nucleus of the solitary tract) MBA:651 projects to VM (Ventral medial nucleus of the thalamus) MBA:685 · Mouse

mCherry-labeled cNTS terminals were observed in the thalamic VM nucleus in cVNS and aVNS mice.

Fig. 9A–B, A1–B1

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