Prelimbic cortex-basolateral amygdala glutamatergic circuit drives cancer-induced bone pain in mice

PL (Prelimbic area) MBA:972 synapses onto BLA glutamatergic principal neurons nt-wprma5fcrr · Mouse

An anterograde trans-synaptic AAV2/1-hSyn-Cre injected in the PrL combined with a Cre-dependent VGluT2-driven reporter in the BLA yielded many EYFP-labelled BLA glutamatergic neurons, indicating monosynaptic PrL input onto BLA glutamatergic cells.

Fig. 4C

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PL (Prelimbic area) MBA:972 functionally connects to BLA glutamatergic principal neurons nt-wprma5fcrr · Mouse

Optogenetic activation of PrL glutamatergic axon terminals in the BLA lowered mechanical thresholds in control mice, and this effect was abolished when BLA glutamatergic neurons were chemogenetically silenced; conversely, silencing PrL terminals in BLA raised thresholds in tumour-bearing mice and this was reversed by chemogenetic excitation of BLA glutamatergic neurons. A glutamate sensor in the BLA showed that chemogenetic inhibition of PrL VGluT2+ neurons reduced evoked glutamate release in the BLA, indicating an excitatory PrL-to-BLA functional connection.

Fig. 6A–6G

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PL (Prelimbic area) MBA:972 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

After anterograde AAV2/9-hSyn-EGFP injection into the prelimbic cortex, labelled axon terminals were abundant in the basolateral amygdala.

Fig. 4B

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PL (Prelimbic area) MBA:972 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

A retrograde AAV (AAV2/R-hSyn-EGFP) injected into the BLA produced labelled cell bodies in the prelimbic cortex, indicating that PrL neurons send axons to the BLA.

Fig. 4A

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.