Whole-brain input mapping of the lateral versus medial anterodorsal bed nucleus of the stria terminalis in the mouse

PA (Posterior amygdalar nucleus) MBA:780 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

CAV2-Cre and rabies injections in lateral adBNST retrogradely labelled neurons in the amygdalohippocampal transition area.

Fig. 2M–P; Fig. 6I–L; Table 1

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PAA (Piriform-amygdalar area) MBA:788 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Amygdalopiriform transition area neurons were retrogradely labelled from lateral adBNST with both CAV2-Cre and rabies.

Fig. 2O–P; Fig. 6K–L; Table 1

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PVH (Paraventricular hypothalamic nucleus) MBA:38 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Paraventricular hypothalamic neurons were labelled from medial adBNST but not from lateral adBNST.

Table 2; Table 3

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BLA (Basolateral amygdalar nucleus) MBA:295 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

The basolateral amygdala was a major source of retrogradely labelled inputs to medial adBNST with both tracers. Labelling was sparse in anterior BLA and concentrated in its posterior part.

Fig. 3G–L; Fig. 7H–M; Table 2

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MEA (Medial amygdalar nucleus) MBA:403 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Medial amygdala input was strongly biased toward medial adBNST, with a significantly larger share than in lateral adBNST.

Results, Quantitative comparisons; Table 2; Table 3

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PAG (Periaqueductal gray) MBA:795 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Medial adBNST injections produced weak labelling in the periaqueductal gray.

Fig. 3Q; Table 2

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PVH (Paraventricular hypothalamic nucleus) MBA:38 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

No paraventricular hypothalamic labelling was counted in any of the lateral adBNST CAV2-Cre cases (0.00%), whereas medial adBNST cases showed labelling there.

Table 1; Table 3

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BLAp (Basolateral amygdalar nucleus, posterior part) MBA:311 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Medial adBNST injections with CAV2-Cre and rabies strongly labelled neurons in the posterior basolateral amygdala.

Fig. 3K–L; Fig. 7J–M

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PA (Posterior amygdalar nucleus) MBA:780 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

AAV-ChR2-Venus injected into the AHiA produced dense Venus-labelled axons within medial adBNST.

Fig. 8A–B

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AI (Agranular insular area) MBA:95 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

CAV2-Cre retrograde tracing from lateral adBNST labelled insular cortex neurons. This input was significantly larger than the insular input to medial adBNST.

Fig. 2E; Table 1; Table 3

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RAmb (Midbrain raphe nuclei) MBA:165 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

CAV2-Cre and rabies retrograde tracing from lateral adBNST labelled neurons in the dorsal raphe and caudal linear raphe nucleus.

Fig. 2R; Fig. 6M–N; Table 1

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BLA (Basolateral amygdalar nucleus) MBA:295 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Lateral adBNST received strong retrogradely labelled input from the basolateral amygdala, including its posterior part, with both CAV2-Cre and rabies. This was the largest single input group.

Fig. 2I–N; Fig. 6G–L; Table 1

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ENT (Entorhinal area) MBA:909 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

CAV2-Cre injected into lateral adBNST labelled neurons in the lateral and medial entorhinal cortex.

Fig. 2Q; Table 1

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PIR (Piriform area) MBA:961 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

An Allen Atlas AAV-EGFP injection in piriform cortex labelled axons in adBNST. Some of the examined cases showed relatively weak labelling.

Fig. 9C

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BLA (Basolateral amygdalar nucleus) MBA:295 functionally connects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Photoactivating BLA ChR2-Venus axon terminals in BNST slices evoked inhibitory postsynaptic currents in recorded BNST neurons, which the paper interprets as an inhibitory input.

Fig. 8F

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COA (Cortical amygdalar area) MBA:631 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Cortical amygdaloid neurons, including the posteromedial cortical nucleus, were retrogradely labelled from lateral adBNST.

Fig. 2M–P; Table 1

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CA (Ammon's horn) MBA:375 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Hippocampal neurons, including CA1, were labelled from medial adBNST at a fair density. The share was significantly larger than for lateral adBNST.

Fig. 7N–P; Table 2; Table 3

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AI (Agranular insular area) MBA:95 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Insular cortex contributed only a small share of CAV2-Cre inputs to medial adBNST, significantly less than to lateral adBNST.

Table 2; Table 3

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VMPO (Ventromedial preoptic nucleus) MBA:576073699 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Rabies retrograde tracing from medial adBNST labelled neurons in the ventromedial preoptic nucleus.

Fig. 7C–E

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AM (Anteromedial nucleus) MBA:127 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

An Allen Atlas AAV anterograde case in the anteromedial thalamus showed axonal labelling in adBNST.

Fig. 9B

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ECT (Ectorhinal area) MBA:895 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

CAV2-Cre injection into lateral adBNST labelled neurons in posterior ectorhinal cortex.

Fig. 2F

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ENT (Entorhinal area) MBA:909 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Entorhinal neurons, notably in lateral entorhinal cortex, were retrogradely labelled from medial adBNST at a fair density.

Fig. 3O–P; Fig. 7N–P; Table 2

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DMH (Dorsomedial nucleus of the hypothalamus) MBA:830 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

A small share of CAV2-Cre labelled inputs to lateral adBNST came from the dorsomedial hypothalamus, significantly less than for medial adBNST.

Table 1; Table 3

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CA (Ammon's horn) MBA:375 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Labelled cells in the hippocampus proper contributed a small share of CAV2-Cre inputs to lateral adBNST. This share was significantly smaller than for medial adBNST.

Table 1; Table 3

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ARH (Arcuate hypothalamic nucleus) MBA:223 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Arcuate input to medial adBNST was significantly larger than to lateral adBNST in CAV2-Cre tracing.

Table 2; Table 3

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HY (Hypothalamus) MBA:1097 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

The hypothalamic preoptic region was strongly labelled from medial adBNST, significantly more than from lateral adBNST.

Results, Overall input patterns; Table 2; Table 3

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BLA (Basolateral amygdalar nucleus) MBA:295 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

AAV-ChR2-Venus injected into the BLA labelled axons that overlapped the BNST around the recorded cell.

Fig. 8D–E

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CEA (Central amygdalar nucleus) MBA:536 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Medial adBNST injections labelled neurons in several central amygdala subdivisions (CeMAD, CeMAV, CeC), with both CAV2-Cre and rabies.

Fig. 3G–J; Fig. 7H–I; Table 2

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RAmb (Midbrain raphe nuclei) MBA:165 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

A small number of dorsal raphe and caudal linear raphe neurons were retrogradely labelled from medial adBNST.

Table 2

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PL (Prelimbic area) MBA:972 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

CAV2-Cre injected into lateral adBNST of Ai9 mice gave intense retrograde labelling in prelimbic cortex, and rabies tracing confirmed it. The combined AC/PL/IL group held about 9.05% (SE 1.78) of all labelled inputs.

Fig. 2A–D; Fig. 6C–D; Table 1

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PA (Posterior amygdalar nucleus) MBA:780 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

CAV2-Cre and rabies injected into medial adBNST strongly labelled the amygdalohippocampal transition area.

Fig. 3K–L; Fig. 7J–K; Table 2

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LHA (Lateral hypothalamic area) MBA:194 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Medial adBNST injections labelled lateral hypothalamic neurons at a fair density.

Fig. 3E–F; Fig. 7F–G; Table 2

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BMA (Basomedial amygdalar nucleus) MBA:319 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Neurons in the anterior basomedial amygdala were retrogradely labelled from medial adBNST.

Fig. 3G–J; Fig. 7H–I

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SUB (Subiculum) MBA:502 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Medial adBNST received fairly dense subicular input with both tracers, significantly more than lateral adBNST.

Fig. 3O–P; Fig. 7N–P; Table 2; Table 3

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AM (Anteromedial nucleus) MBA:127 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Anterior thalamic neurons were labelled after CAV2-Cre injection into medial adBNST, though significantly fewer than for lateral adBNST.

Table 2; Table 3

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ILA (Infralimbic area) MBA:44 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Retrograde labelling from medial adBNST in infralimbic cortex was sparse.

Fig. 3A–B

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VMH (Ventromedial hypothalamic nucleus) MBA:693 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Few labelled cells in the ventromedial hypothalamus were found after CAV2-Cre injection into lateral adBNST, significantly fewer than for medial adBNST.

Table 1; Table 3

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PA (Posterior amygdalar nucleus) MBA:780 functionally connects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

In slices bathed in TTX and 4-AP, laser photostimulation of AHiA ChR2 axons evoked excitatory postsynaptic currents in medial adBNST neurons recorded by whole-cell patch clamp.

Fig. 8C

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AHN (Anterior hypothalamic nucleus) MBA:88 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Anterior hypothalamic neurons were retrogradely labelled after CAV2-Cre injection into medial adBNST.

Table 2

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PAA (Piriform-amygdalar area) MBA:788 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Amygdalopiriform transition area neurons were labelled after CAV2-Cre injection into medial adBNST.

Fig. 3M–P; Table 2

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LS (Lateral septal nucleus) MBA:242 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Only a small share of CAV2-Cre inputs to lateral adBNST came from the lateral septum. This share was significantly lower than for medial adBNST.

Table 1; Table 3

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ACA (Anterior cingulate area) MBA:31 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

CAV2-Cre injected into medial adBNST labelled only a few neurons in the anterior cingulate cortex.

Fig. 3C–D

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PERI (Perirhinal area) MBA:922 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

An Allen Atlas AAV injection covering ectorhinal and perirhinal cortex produced axonal labelling in adBNST.

Fig. 9D

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CM (Central medial nucleus of the thalamus) MBA:599 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Rabies retrograde tracing from lateral adBNST labelled neurons in the central medial thalamic nucleus.

Fig. 6E–F

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COA (Cortical amygdalar area) MBA:631 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Cortical amygdaloid neurons, including the posteromedial cortical nucleus, were retrogradely labelled from medial adBNST.

Fig. 3M–N; Fig. 7N–P; Table 2

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SCdg (Superior colliculus, motor related, deep gray layer) MBA:26 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

No labelled neurons in the deep gray layer of the superior colliculus were counted after CAV2-Cre injection into lateral adBNST. The paper states that this input reaches medial but not lateral adBNST.

Fig. 5; Table 1; Table 3

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LS (Lateral septal nucleus) MBA:242 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Medial adBNST injections with CAV2-Cre and rabies strongly labelled lateral septal neurons. This input was significantly larger than to lateral adBNST.

Results, Overall input patterns; Fig. 7C–E; Table 2; Table 3

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CEA (Central amygdalar nucleus) MBA:536 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Neurons in the lateral and medial central amygdala were retrogradely labelled from lateral adBNST with both CAV2-Cre and rabies.

Fig. 2I–L; Fig. 6G–H; Table 1

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PERI (Perirhinal area) MBA:922 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Few perirhinal neurons were labelled from medial adBNST, significantly fewer than from lateral adBNST.

Table 2; Table 3

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VMH (Ventromedial hypothalamic nucleus) MBA:693 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Ventromedial hypothalamic neurons were retrogradely labelled from medial adBNST, significantly more than from lateral adBNST.

Table 2; Table 3

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