One-step optogenetics with multifunctional flexible polymer fibers

BLA (Basolateral amygdalar nucleus) MBA:295 projects to CA2 (Field CA2) MBA:423 · Mouse

Six weeks after injecting AAV5-CaMKIIα::ChR2-eYFP into the BLA through the fiber probe, eYFP-labelled fibers were imaged in the CA2 region of the ventral hippocampus.

Fig. 3f, g

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 functionally connects to CA (Ammon's horn) MBA:375 · Mouse

Light pulses delivered in the ventral hippocampus after ChR2 expression in the BLA produced only short-latency evoked potentials, consistent with direct axonal input, detectable about 11 days after injection. Local infusion of the AMPA-receptor antagonist CNQX through the probe suppressed the optically evoked response and abolished the stimulation-induced behavioural change, indicating a glutamatergic synaptic connection.

Fig. 3k, 3o; Supplementary Fig. 10

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) is unsure. “Short-latency vHPC responses and the CNQX block of the behavioural effect are reported, but the text never states that CNQX suppressed the evoked potentials, which leaves the excitatory sign only indirectly supported.”

BLA (Basolateral amygdalar nucleus) MBA:295 projects to PL (Prelimbic area) MBA:972 · Mouse

AAV5-CaMKIIα::ChR2-eYFP was infused into the BLA through the fiber probe's microfluidic channel; six weeks later eYFP-labelled axons were visible in coronal sections of the medial prefrontal cortex, including the prelimbic subdivision.

Fig. 3b–d

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 projects to ILA (Infralimbic area) MBA:44 · Mouse

Following viral delivery of ChR2-eYFP into the BLA, fluorescent axonal labelling was imaged in the infralimbic subdivision of the medial prefrontal cortex.

Fig. 3b, d

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 projects to CA3 (Field CA3) MBA:463 · Mouse

Labelled axons from BLA neurons transduced with ChR2-eYFP were also seen in the CA3 region of the ventral hippocampus in coronal sections.

Fig. 3f, h

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 functionally connects to PL (Prelimbic area) MBA:972 · Mouse

After expressing ChR2 in BLA neurons, blue-light pulses delivered through a probe in the mPFC evoked local field responses at a short latency (~5 ms after each pulse), appearing about 12 days post-injection; a second, longer-latency (~32 ms) component appeared later and was attributed by the authors to polysynaptic network activity within the mPFC.

Fig. 3j; Results, projection mapping section

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) disagrees. “The paper records from and names only the mPFC, never the prelimbic area specifically, so the object region is narrower than the paper's own region.”