Constitutive 5-HT2C receptor knock-out facilitates fear extinction through altered activity of a dorsal raphe-bed nucleus of the stria terminalis pathway

BST (Bed nuclei of the stria terminalis) MBA:351 projects to DR (Dorsal nucleus raphe) MBA:872 · Mouse

Cre-dependent tdTomato AAV injected into the anterodorsal BNST of Gad2-Ires-Cre mice labelled GABAergic terminals at all rostrocaudal levels of the dorsal raphe. Rostral and medial levels were innervated significantly more strongly than caudal levels (n = 6 mice).

Fig. 4a, f-h

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

BST (Bed nuclei of the stria terminalis) MBA:351 projects to DR (Dorsal nucleus raphe) MBA:872 · Mouse

Fluorogold injected into the medial dorsal raphe retrogradely labelled cells mainly in the anterodorsal BNST. Only a small number of labelled cells were in the oval nucleus.

Fig. 5a, e-f

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

DR (Dorsal nucleus raphe) MBA:872 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Cre-dependent tdTomato AAV injected into the caudal dorsal raphe of ePet1-Cre mice labelled serotonergic terminals in the dorsal BNST bilaterally. Terminal fluorescence was significantly higher in the anterodorsal BNST than in the oval nucleus (n = 3 mice).

Fig. 4a-e

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

DR (Dorsal nucleus raphe) MBA:872 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Fluorogold injected into the anterodorsal BNST densely labelled TPH2-positive serotonergic cells in the caudal dorsal raphe, with only a few labelled cells at other raphe levels. This confirms a discrete serotonergic DRC-to-BNSTad projection.

Fig. 5a, c-d; Supplementary Fig. 3

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.