Determinants of functional synaptic connectivity among amygdala-projecting prefrontal cortical neurons in male mice

ACA (Anterior cingulate area) MBA:31 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

BLA-projecting neurons were retrogradely labelled in the dorsal mPFC, which includes the cingulate cortex, and were largely absent from layer 3.

Fig. 1b, Fig. S1a, Fig. S6

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ILA2/3 (Infralimbic area, layer 2/3) MBA:556 functionally connects to ILA5 (Infralimbic area, layer 5) MBA:827 · Mouse

Among BLA-projecting ventral mPFC cells, stimulating layer 2 and layer 3 cells produced connections onto layer 5 BLA-projecting cells.

Fig. 3d

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DP (Dorsal peduncular area) MBA:814 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Retrograde AAV-Cre in the BLA labelled dorsal peduncular neurons, which were densely spread across all layers with a laminar pattern like the infralimbic cortex.

Fig. 1b, Fig. S1a, Fig. S6

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ILA2/3 (Infralimbic area, layer 2/3) MBA:556 functionally connects to ILA5 (Infralimbic area, layer 5) MBA:827 · Mouse

No input from layer 2 or 3 random cells was detected onto layer 5 random cells, nor from layer 2/3 mPFC-BLA cells onto layer 5 non-mPFC-BLA cells. The authors note this may reflect the small number of such maps.

Fig. 3d

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ILA (Infralimbic area) MBA:44 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

rAAV2-retro-Cre injected into the BLA of Ai9 or wildtype mice labelled many infralimbic neurons, which were densely distributed across all cortical layers.

Fig. 1b, Fig. S1a, Fig. S6

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PL (Prelimbic area) MBA:972 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Retrograde labelling from the BLA marked neurons in the dorsal mPFC, including the prelimbic cortex. These BLA-projecting cells were largely missing from layer 3.

Fig. 1b, Fig. S1a, Fig. S6

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.