A quadruple dissociation of reward-related behaviour in mice across excitatory inputs to the nucleus accumbens shell

ILA (Infralimbic area) MBA:44 projects to ACB (Nucleus accumbens) MBA:56 · Mouse

AAV2-CaMKIIa-ChR2-eYFP injected into the infralimbic medial prefrontal cortex produced eYFP-labelled axon terminals in the nucleus accumbens shell, where optical fibres were placed over the labelled terminal field.

Fig. 1b,c; Supplementary Fig. 1b,c; Methods (Surgical procedures)

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

HPF (Hippocampal formation) MBA:1089 projects to ACB (Nucleus accumbens) MBA:56 · Mouse

Viral expression of ChR2-eYFP under the CaMKIIa promoter in the ventral hippocampus labelled terminals in the nucleus accumbens shell, which were targeted by implanted optical fibres.

Fig. 1b,c; Supplementary Fig. 1b,c; Methods (Surgical procedures)

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 projects to ACB (Nucleus accumbens) MBA:56 · Mouse

CaMKIIa-driven ChR2-eYFP expression in the basolateral amygdala yielded labelled axon terminals in the nucleus accumbens shell under the implanted fibre tips.

Fig. 1b,c; Supplementary Fig. 1b,c; Methods (Surgical procedures)

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PVT (Paraventricular nucleus of the thalamus) MBA:149 projects to ACB (Nucleus accumbens) MBA:56 · Mouse

Injection of AAV2-CaMKIIa-ChR2-eYFP into the paraventricular thalamic nucleus labelled terminals in the nucleus accumbens shell, verified histologically beneath the optical fibre placements.

Fig. 1b,c; Supplementary Fig. 1b,c; Methods (Surgical procedures)

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.