Synaptic potentiation of engram cells is necessary and sufficient for context fear memory

PL (Prelimbic area) MBA:972 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

A retrogradely transported AAV2-retro carrying a Cre-dependent ChR2-EYFP was injected into the BLA; among the labelled input regions the prefrontal cortex, including its prelimbic part, showed the strongest expression, indicating prelimbic neurons send axons to BLA.

Fig. 3B; Supplementary Fig. 7

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PAG (Periaqueductal gray) MBA:795 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Periaqueductal gray showed minimal to no retrograde labelling from BLA with the AAV2-retro vector, interpreted as a limitation of the serotype for subcortical afferents.

Supplementary Fig. 7E

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

ENTl (Entorhinal area, lateral part) MBA:918 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

BLA injection of the retrograde AAV2-retro vector labelled neurons in lateral entorhinal cortex, ranked second in labelling intensity after prefrontal cortex.

Fig. 3B; Supplementary Fig. 7

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PERI (Perirhinal area) MBA:922 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Perirhinal cortical neurons were retrogradely labelled after BLA injection of AAV2-retro, placing them among the BLA input regions.

Fig. 3B; Supplementary Fig. 7

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 projects to ACB (Nucleus accumbens) MBA:56 · Mouse

Synaptophysin-Venus labelling of tagged BLA neurons revealed terminals in the nucleus accumbens core, with denser labelling from neurons tagged during context exposure alone than from those tagged during context conditioning.

Supplementary Fig. 10

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PVT (Paraventricular nucleus of the thalamus) MBA:149 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Retrograde labelling from the BLA injection appeared in the paraventricular thalamic nucleus, indicating a PVT input to BLA.

Fig. 3B; Supplementary Fig. 7

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

ECT (Ectorhinal area) MBA:895 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Ectorhinal cortical neurons were retrogradely labelled by the AAV2-retro vector injected in BLA.

Fig. 3B; Supplementary Fig. 7

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

AUD (Auditory areas) MBA:247 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Retrograde labelling of auditory cortex from BLA injections was inconsistent and weak, appearing only when the injection reached more dorsal BLA, so the result did not settle the question.

Fig. 3B

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 projects to PL (Prelimbic area) MBA:972 · Mouse

Terminal fields of activity-tagged BLA neurons labelled with synaptophysin-Venus were measured in the prefrontal cortex as well as in central amygdala and accumbens, showing a BLA axonal projection to prefrontal cortex.

Supplementary Fig. 10 (Methods, synaptophysin-Venus quantification)

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) is unsure. “Only Methods mention measuring Syn-Venus signal in PFC; no result for BLA→PFC is reported in the text.”

HIP (Hippocampal region) MBA:1080 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

After BLA injection of AAV2-retro only minimal or no labelling was seen in ventral hippocampus; the authors attribute this to the serotype's preference for cortical inputs rather than to a lack of projection.

Supplementary Fig. 7E

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

ILA (Infralimbic area) MBA:44 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

AAV2-retro injected in BLA labelled infralimbic cortical neurons, part of the prefrontal region with the most intense retrograde expression, showing an infralimbic projection to BLA.

Fig. 3B; Supplementary Fig. 7

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 projects to CEA (Central amygdalar nucleus) MBA:536 · Mouse

Cre-dependent synaptophysin-Venus expressed in activity-tagged BLA neurons produced axonal terminal labelling in the central amygdala; the labelled area was smaller for neurons tagged during context exposure alone than for neurons tagged during context conditioning.

Supplementary Fig. 10

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

ORBm (Orbital area, medial part) MBA:731 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Retrograde AAV2-retro delivered to BLA produced labelling in the medial orbital cortex, counted by the authors among the prefrontal regions with the strongest expression.

Fig. 3B; Supplementary Fig. 7

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

AI (Agranular insular area) MBA:95 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Insular cortex showed retrograde labelling, the least intense among the clearly labelled input regions, after AAV2-retro injection into BLA.

Fig. 3B; Supplementary Fig. 7

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

MG (Medial geniculate complex) MBA:475 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

The medial geniculate nucleus showed minimal to no retrograde labelling after BLA injection of AAV2-retro, which the authors ascribe to poor infection of subcortical inputs by this serotype.

Supplementary Fig. 7E

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

ENTm (Entorhinal area, medial part, dorsal zone) MBA:926 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Medial entorhinal neurons were retrogradely labelled after AAV2-retro injection into BLA.

Fig. 3B; Supplementary Fig. 7

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.