Dopamine D2 receptor-mediated circuit from the central amygdala to the bed nucleus of the stria terminalis regulates impulsive behavior

CEA (Central amygdalar nucleus) MBA:536 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Cholera toxin B injected into the BNST of D2R-EGFP mice labelled CeA neurons, with roughly 59% of the EGFP-positive CeA cells also tracer-positive; DiI injections into BNST gave similar results.

Fig. 3 D and E; SI Appendix, Fig. S9

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CEA (Central amygdalar nucleus) MBA:536 projects to MD (Mediodorsal nucleus of thalamus) MBA:362 · Mouse

ChR2-EYFP-labelled axons from D2R-expressing CeA neurons were also found in the mediodorsal thalamus.

SI Appendix, Fig. S8 A and B (described in Results)

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CEA (Central amygdalar nucleus) MBA:536 functionally connects to CL:0000617 · Mouse

Post hoc staining of light-responsive BNST neurons showed that 7 of 10 cells with photostimulation-evoked IPSCs were VGAT-positive, indicating that GABAergic BNST neurons receive inhibitory CeA D2R input; IPSC amplitudes did not differ from VGAT-negative cells.

Fig. 5 A–D

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CEA (Central amygdalar nucleus) MBA:536 functionally connects to CL:0000617 · Mouse

Of nine recorded VTA neurons shown to be VGAT-positive, five received light-evoked inhibitory input from stimulated CeA D2R terminals.

Fig. 4 C and D

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CEA (Central amygdalar nucleus) MBA:536 functionally connects to CL:0000679 · Mouse

Three of the light-responsive BNST neurons were VGlut2-positive, showing that glutamatergic BNST cells also receive inhibitory input from CeA D2R neurons.

Fig. 5 B–D

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CEA (Central amygdalar nucleus) MBA:536 functionally connects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Light activation of ChR2-expressing CeA D2R axons in BNST slices elicited inhibitory postsynaptic currents in 31 of 45 recorded BNST neurons in the lateral posterior and ventral subregions.

Fig. 5 B, C and D

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CEA (Central amygdalar nucleus) MBA:536 projects to LHA (Lateral hypothalamic area) MBA:194 · Mouse

ChR2-EYFP-expressing axons of CeA D2R neurons reached the lateral hypothalamus.

SI Appendix, Fig. S8 A and B (described in Results)

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CEA (Central amygdalar nucleus) MBA:536 functionally connects to VTA (Ventral tegmental area) MBA:749 · Mouse

Photostimulating ChR2-expressing CeA D2R axons in VTA slices evoked inhibitory postsynaptic currents in 41% of recorded VTA neurons, including both Ih-positive and Ih-negative cells.

Fig. 4 A, B and D

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CEA (Central amygdalar nucleus) MBA:536 projects to SNc (Substantia nigra, compact part) MBA:374 · Mouse

Axons of ChR2-EYFP-labelled CeA D2R neurons were detected in the substantia nigra, where the compact part is identified in the imaged field.

Fig. 3A; SI Appendix, Fig. S8

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CEA (Central amygdalar nucleus) MBA:536 projects to VTA (Ventral tegmental area) MBA:749 · Mouse

After Cre-dependent ChR2-EYFP injection into the CeA of D2R-Cre mice, fluorescent axon bundles were seen in the ventral tegmental area.

Fig. 3A

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CEA (Central amygdalar nucleus) MBA:536 projects to SNr (Substantia nigra, reticular part) MBA:381 · Mouse

Axons of ChR2-EYFP-labelled CeA D2R neurons were detected in the substantia nigra, where the reticular part is identified in the imaged field.

Fig. 3A; SI Appendix, Fig. S8

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CEA (Central amygdalar nucleus) MBA:536 functionally connects to CL:0000700 · Mouse

Among recorded VTA neurons later identified as tyrosine hydroxylase-positive, half (6 of 12) showed light-evoked inhibitory currents when CeA D2R terminals were stimulated.

Fig. 4 C and D

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CEA (Central amygdalar nucleus) MBA:536 projects to VTA (Ventral tegmental area) MBA:749 · Mouse

Cholera toxin B injected into the VTA of D2R-EGFP mice retrogradely labelled CeA neurons, with about 63% of EGFP-positive CeA cells containing the tracer; injection of DiI into the VTA gave comparable labelling.

Fig. 3 B and C; SI Appendix, Fig. S9

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CEA (Central amygdalar nucleus) MBA:536 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Cre-dependent ChR2-EYFP expressed in D2R-positive CeA neurons produced labelled axons in the BNST, mainly in its ventrolateral, ventromedial and lateral posterior parts.

Fig. 3A; SI Appendix, Fig. S11A

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CEA (Central amygdalar nucleus) MBA:536 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Labelled fibres from D2R-positive CeA neurons expressing ChR2-EYFP were observed in the basolateral amygdala.

SI Appendix, Fig. S8 A and B (described in Results)

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