A network of basolateral amygdala projection neurons contributes to stress-induced activation of the hypothalamic-pituitary-adrenal axis

BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 projects to PL (Prelimbic area) UBERON:8440032 · Rat

Bilateral CTB injection into the prelimbic cortex retrogradely labelled BLA neurons, most of which lay in the medial basal subdivision; retrograde AAV-Cre in PrL combined with a Cre-dependent virus in BLA likewise labelled this population.

Fig. 4, A to M; fig. S5B

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 projects to HPF (Hippocampal formation) UBERON:0002421 · Rat

CTB in the ventral hippocampus labelled BLA neurons distributed in the medial basal amygdala and the lateral amygdala.

Fig. 4, A to M

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 projects to ACB (Nucleus accumbens) UBERON:0001882 · Rat

CTB placed in the nucleus accumbens labelled a dense population of BLA neurons biased toward the medial basal amygdala; density of accumbens projectors was higher than that of PrL, LH and VH projectors.

Fig. 4, A to M; fig. S5B

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 functionally connects to CEA (central amygdaloid nucleus) UBERON:0002883 · Rat

Central amygdala FOS density was measured after photostimulation of BLA CaMKII neurons and showed no significant increase over control animals.

Fig. 2J

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 projects to ACB (Nucleus accumbens) UBERON:0001882 · Rat

CaMKII-driven eGFP delivered to the BLA produced pronounced fibre labelling in the nucleus accumbens.

Fig. 3, A to H; fig. S3

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 projects to CEA (central amygdaloid nucleus) UBERON:0002883 · Rat

eGFP-labelled BLA axons were abundant in the central amygdala after BLA injection of AAV8-CaMKII-eGFP.

Fig. 3, A to H; fig. S3

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 projects to BST (Bed nuclei of the stria terminalis) UBERON:0001880 · Rat

Anterograde viral labelling of BLA CaMKII neurons revealed marked axonal eGFP in the bed nucleus of the stria terminalis.

Fig. 3, A to H; fig. S3

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 projects to CEA (central amygdaloid nucleus) UBERON:0002883 · Rat

CTB injected into the central amygdala labelled BLA neurons located mainly in the lateral amygdala and lateral basal amygdala, with few in the medial basal subdivision.

Fig. 4, A to M; fig. S5B

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 projects to BST (Bed nuclei of the stria terminalis) UBERON:0001880 · Rat

CTB in the bed nucleus of the stria terminalis retrogradely labelled BLA neurons concentrated in the medial basal amygdala, at higher density than PrL-, LH- or VH-projecting cells.

Fig. 4, A to M; fig. S5B

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 projects to LHA (Lateral hypothalamic area) UBERON:0002430 · Rat

CTB in the lateral hypothalamus retrogradely labelled BLA cells, most of them residing in the lateral basal amygdala and few in the medial basal subdivision.

Fig. 4, A to M

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 functionally connects to BST (Bed nuclei of the stria terminalis) UBERON:0001880 · Rat

Optogenetic activation of BLA projection neurons increased FOS in the dorsomedial subdivision of the bed nucleus of the stria terminalis, whereas the anterodorsal subdivision showed no significant increase.

Fig. 2, J and K

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 functionally connects to PL (Prelimbic area) UBERON:8440032 · Rat

Photostimulation of ChR2-expressing BLA CaMKII neurons (20 Hz, 15 min) raised FOS density in the prelimbic cortex relative to mCherry controls.

Fig. 2, J and K

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 functionally connects to PVH (Paraventricular hypothalamic nucleus) UBERON:0001930 · Rat

Photostimulating BLA CaMKII neurons increased FOS density in the paraventricular hypothalamic nucleus compared with mCherry controls; the authors interpret this as indirect activation since the BLA lacks a direct PVN projection.

Fig. 2, H and I

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 functionally connects to ACB (Nucleus accumbens) UBERON:0001882 · Rat

FOS density in the core and shell of the nucleus accumbens was quantified after optogenetic stimulation of BLA projection neurons but did not differ significantly from mCherry controls.

Fig. 2J

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 functionally connects to HPF (Hippocampal formation) UBERON:0002421 · Rat

Ventral hippocampal FOS density was quantified following BLA optogenetic stimulation and showed no significant change relative to controls.

Fig. 2J

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 projects to HPF (Hippocampal formation) UBERON:0002421 · Rat

Anterograde tracing from the BLA produced strong eGFP fibre labelling in the ventral hippocampus.

Fig. 3, A to H; fig. S3

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 projects to PL (Prelimbic area) UBERON:8440032 · Rat

After injecting AAV8-CaMKII-eGFP into the BLA of three rats, labelled axons were traced through the brain and prominent fibre labelling was found in the prelimbic cortex among six limbic targets.

Fig. 3, A to H; fig. S3

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 functionally connects to LHA (Lateral hypothalamic area) UBERON:0002430 · Rat

Lateral hypothalamic FOS density after BLA photostimulation was not significantly different from mCherry controls.

Fig. 2J

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BLN (basolateral amygdaloid nuclear complex) UBERON:0006107 projects to LHA (Lateral hypothalamic area) UBERON:0002430 · Rat

Fibres from virally labelled BLA CaMKII neurons were seen in the lateral hypothalamus.

Fig. 3, A to H; fig. S3

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.