Gastric vagal afferent signaling to the basolateral amygdala mediates anxiety-like behaviors in experimental colitis mice

NTS (Nucleus of the solitary tract) MBA:651 synapses onto LC (Locus ceruleus) MBA:147 · Mouse

Monosynaptic rabies tracing from noradrenergic LC starter cells in DBH-Cre mice (TVA/RG helpers plus EnvA-dG-mCherry) labelled mCherry-positive presynaptic neurons in the NTS, indicating direct NTS input onto LC noradrenergic neurons.

Fig. 6D, E, G

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LC (Locus ceruleus) MBA:147 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Fluorogold injected into the BLA retrogradely labelled many neurons in the posterior locus coeruleus, and these cells were immunopositive for the norepinephrine transporter.

Fig. 6A–C

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NTS (Nucleus of the solitary tract) MBA:651 projects to LC (Locus ceruleus) MBA:147 · Mouse

After unilateral injection of an anterograde AAV (Ubi-GFP, or CaMKIIa-mCherry in a second cohort) into the NTS, labelled axons were seen in the locus coeruleus, apposed to NET-positive LC cells that had been retrogradely labelled from the BLA.

Fig. 6A–C; Supplemental Fig. 8

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NTS (Nucleus of the solitary tract) MBA:651 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Fluorogold injected into the BLA produced only very sparse retrograde labelling in the NTS, leading the authors to conclude that NTS reaches the BLA mainly indirectly through noradrenergic LC neurons.

Supplemental Fig. 8C; Results, gut-to-brain circuit section

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LC (Locus ceruleus) MBA:147 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Cre-dependent helper AAV (FLEX-TVA-GFP) injected into the LC of DBH-Cre mice produced GFP-labelled axonal fibres in the BLA, showing that noradrenergic LC neurons send axons there.

Fig. 6D–F

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