Characterization of Anxiety-Like Behaviors and Neural Circuitry following Chronic Moderate Noise Exposure in Mice

MG (Medial geniculate complex) MBA:475 synapses onto LA (Lateral amygdalar nucleus) MBA:131 · Mouse

AAV2/1-Flpo injected into the MG spread anterogradely across synapses and, with fDIO-EYFP in the LA, labelled postsynaptic LA neurons. Most of these were glutamatergic, and they overlapped largely with neurons receiving ACx input.

Fig. 4I-L

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AUD (Auditory areas) MBA:247 functionally connects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

In slices, optogenetic stimulation of ChR2-expressing ACx terminals evoked EPSCs and spiking in LA neurons. Outward IPSCs followed at longer latency, indicating disynaptic inhibition.

Fig. 4O-Q,S; Fig. S5

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MGd (Medial geniculate complex, dorsal part) MBA:1072 projects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

After retrograde AAV-EGFP injection into the LA, labelled MG neurons sat mainly in the nonlemniscal nuclei, including the dorsal subdivision.

Fig. 4C

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MG (Medial geniculate complex) MBA:475 functionally connects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

In slices, light stimulation of ChR2-expressing MG fibres in the LA evoked short-latency EPSCs and action potentials in LA neurons. It also evoked longer-latency, disynaptic IPSCs, consistent with monosynaptic excitation plus feedforward inhibition.

Fig. 4M,N,Q,R; Fig. S5

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AUD (Auditory areas) MBA:247 projects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

Retrograde AAV-EGFP injected into the LA labelled auditory cortex neurons, mainly in layers V and VI. Most were glutamate-positive and few were GABA-positive.

Fig. 4F-H

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MGm (Medial geniculate complex, medial part) MBA:1088 projects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

RetroAAV-EGFP injected into the LA labelled neurons in the auditory thalamus, mostly in its nonlemniscal medial and dorsal subdivisions. These labelled neurons were glutamate-immunoreactive and not GABA-immunoreactive.

Fig. 4A-E

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LA (Lateral amygdalar nucleus) MBA:131 functionally connects to LA glutamatergic principal neurons nt-659aayyts5 · Mouse

ChR2 was expressed in Gad2-Cre GABAergic neurons of the LA. Light stimulation of these neurons evoked IPSCs in LA principal neurons recorded in slices.

Fig. S5M-O

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AUD (Auditory areas) MBA:247 synapses onto LA (Lateral amygdalar nucleus) MBA:131 · Mouse

AAV2/1-Cre injected into the ACx crossed synapses and, with DIO-mCherry in the LA, labelled postsynaptic LA neurons. These were predominantly glutamatergic.

Fig. 4I-L

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.