Distribution of VGLUT3 in Highly Collateralized Axons from the Rat Dorsal Raphe Nucleus as Revealed by Single-Neuron Reconstructions

DR (Dorsal nucleus raphe) UBERON:0002043 projects to VTA (Ventral tegmental area) UBERON:0002691 · Rat

A ventral branch of a thalamus-projecting DRN axon innervated the ventral tegmental area.

Fig. 2D; Results, Diencephalon and midbrain tegmentum

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to MBO (Mammillary body) UBERON:0002206 · Rat

A reconstructed DRN axon branch innervated the mammillary body.

Fig. 2D; Results, Diencephalon and midbrain tegmentum

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to MO (Somatomotor areas) UBERON:8440076 · Rat

A reconstructed DRN axon entered the motor cortex and split into thin collaterals in all layers. In confocal analysis, 75% of 259 BDA-filled varicosities from 29 segments in motor cortex contained VGLUT3.

Fig. 2B; Figs. 5-7; Results

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to LS (Lateral septal nucleus) UBERON:0002667 · Rat

A prefrontal-projecting DRN axon entered the lateral septum and left a small number of varicosities there.

Fig. 3C; Results, Prefrontal cortex

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to PCN (Paracentral nucleus) UBERON:0001924 · Rat

A dorsal branch of a reconstructed DRN axon arborized in the paracentral thalamic nucleus.

Fig. 2D; Results, Diencephalon and midbrain tegmentum

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to CA2 (Field CA2) UBERON:0003882 · Rat

The same reconstructed DRN hippocampal collateral also arborized in CA2.

Fig. 3C; Results, Prefrontal cortex

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to PF (Parafascicular nucleus) UBERON:0001922 · Rat

A reconstructed DRN axon arborized in the parafascicular thalamic nucleus.

Fig. 2D; Results, Diencephalon and midbrain tegmentum

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to BST (Bed nuclei of the stria terminalis) UBERON:0001880 · Rat

Amygdala-projecting DRN axons entered the bed nucleus of the stria terminalis, and one neuron innervated it profusely.

Fig. 3A, 3B; Results, Amygdala

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to ACB (Nucleus accumbens) UBERON:0001882 · Rat

Several reconstructed DRN axons terminated in the nucleus accumbens. One formed a wide, dense terminal arborization there.

Fig. 2C, 2D, Fig. 3A, 3D; Results

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to SNc (Substantia nigra, compact part) UBERON:0001965 · Rat

Reconstructed DRN axons crossed the substantia nigra pars compacta bearing en passant varicosities, and one neuron arborized there.

Fig. 2B, 2E; Results, Striatum and Diencephalon and midbrain tegmentum

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to LHA (Lateral hypothalamic area) UBERON:0002430 · Rat

Most DRN axons ascended through the lateral hypothalamic area with beaded segments. Some neurons gave beaded collaterals there or innervated it directly.

Fig. 2C, 2D; Results, Axonal trajectory

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to STRd (Striatum dorsal region) UBERON:0005382 · Rat

Individually BDA-filled DRN axons were traced into the dorsolateral (sensorimotor) striatum, where they broke into many terminal collaterals. One axon bore about 2,131 varicosities there.

Fig. 2A, 2B; Results, Striatum

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to STN (Subthalamic nucleus) UBERON:0001906 · Rat

One reconstructed DRN neuron sent an axon that arborized in the subthalamic nucleus.

Fig. 2E; Results, Diencephalon and midbrain tegmentum

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to CA1 (Field CA1) UBERON:0003881 · Rat

A collateral of a reconstructed DRN axon entered the hippocampal formation and arborized profusely in CA1.

Fig. 3C; Results, Prefrontal cortex

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to CA3 (Field CA3) UBERON:0003883 · Rat

The same reconstructed DRN hippocampal collateral also arborized in CA3.

Fig. 3C; Results, Prefrontal cortex

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to OT (Olfactory tubercle) UBERON:0001883 · Rat

Two ventral branches of a reconstructed striatum-projecting DRN axon innervated the olfactory tubercle.

Fig. 2A; Results, Striatum

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to DG (Dentate gyrus) UBERON:0001885 · Rat

The same reconstructed DRN hippocampal collateral also arborized in the dentate gyrus.

Fig. 3C; Results, Prefrontal cortex

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to BLA (basal amygdaloid nucleus) UBERON:0002887 · Rat

Single reconstructed DRN neurons gave terminal arborizations that profusely innervated the basolateral amygdaloid nucleus.

Fig. 3A, 3B; Results, Amygdala

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to CTX (Cerebral cortex) UBERON:0000956 · Rat

Reconstructed DRN axons reached the prefrontal cortex and arborized in all six layers. Varicosity counts per axon ranged from 239 to 3,063.

Fig. 2A, Fig. 3C, 3D; Results, Prefrontal cortex

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to LH (Lateral habenula) UBERON:0001941 · Rat

A reconstructed DRN axon arborized in the lateral habenula.

Fig. 2D; Results, Diencephalon and midbrain tegmentum

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to SUM (Supramammillary nucleus) UBERON:0001940 · Rat

A reconstructed DRN axon branch innervated the supramammillary nucleus.

Fig. 2D; Results, Diencephalon and midbrain tegmentum

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

DR (Dorsal nucleus raphe) UBERON:0002043 projects to PALv (Pallidum, ventral region) UBERON:0002778 · Rat

Labelled DRN axons were seen in the ventral pallidum. One reconstructed axon had only a few varicosities there, and another innervated it alongside the amygdala.

Fig. 1D, Fig. 2B, Fig. 3B; Results

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) is unsure. “The Fig. 2B axon has few varicosities in the ventral pallidum, but the Fig. 3B axon appears to innervate it profusely, so the 'weak' strength is doubtful.”

DR (Dorsal nucleus raphe) UBERON:0002043 projects to CEA (central amygdaloid nucleus) UBERON:0002883 · Rat

Single reconstructed DRN neurons gave terminal arborizations that profusely innervated the central amygdaloid nucleus.

Fig. 3A, 3B; Results, Amygdala

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.