The Bed Nucleus of the Stria Terminalis–Deep Mesencephalic Nucleus Circuit Linking Emotion and Wakefulness

BST (Bed nuclei of the stria terminalis) MBA:351 synapses onto MRN (Midbrain reticular nucleus) MBA:128 · Mouse

Monosynaptic rabies tracing from Vgat-expressing DpMe starter cells labelled Vgat-positive input neurons in the BNST.

Fig. 6C-D; Results, final section

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BST (Bed nuclei of the stria terminalis) MBA:351 synapses onto MRN (Midbrain reticular nucleus) MBA:128 · Mouse

Rabies tracing from Vglut2-expressing DpMe starter cells labelled almost no input neurons in the BNST, so the authors conclude BNST GABAergic neurons do not directly innervate glutamatergic DpMe cells.

Fig. S1; Fig. 6B; Results, final section

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BST (Bed nuclei of the stria terminalis) MBA:351 functionally connects to RR (Midbrain reticular nucleus, retrorubral area) MBA:246 · Mouse

After optogenetic stimulation of GABAergic BNST neurons, tyrosine hydroxylase-positive A8 neurons showed no c-fos mRNA, whereas nearby TH-negative cells were labelled.

Fig. 1I; Results, section 1

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BST (Bed nuclei of the stria terminalis) MBA:351 functionally connects to MRN (Midbrain reticular nucleus) MBA:128 · Mouse

Optogenetic activation of GABAergic BNST neurons during NREM sleep raised the density of c-fos mRNA-positive DpMe cells (about 70 vs 27 cells/mm2) and evoked a transient calcium increase in glutamatergic DpMe neurons measured by fiber photometry.

Fig. 1G-H; Fig. 3D

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BST (Bed nuclei of the stria terminalis) MBA:351 projects to MRN (Midbrain reticular nucleus) MBA:128 · Mouse

Cre-dependent ChR2-EYFP injected into the BNST of Vgat-ires-Cre mice labelled dense GABAergic BNST axons within the DpMe.

Results, section 1; Fig. 1H; Fig. 2B; Fig. 5B

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