Virus Injection to the Pituitary via Transsphenoidal Approach and the Innervation of Anterior and Posterior Pituitary of Rat

CA1 (Field CA1) UBERON:0003881 synapses onto IB (Interbrain) UBERON:0001894 · Rat

Four days after PRV 152 was placed in the posterior lobe of the pituitary, EGFP-positive somata were seen in hippocampal field CA1; because the tracer crosses synapses, the authors describe these regions as directly or indirectly linked to the posterior lobe, so a monosynaptic link is not established.

Fig. 4A,B

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root UBERON:0000955 synapses onto IB (Interbrain) UBERON:0001894 · Rat

PRV 152 expressing EGFP was injected into the anterior lobe and animals were examined daily from 1 to 18 days afterwards; no labelled neurons appeared anywhere in the brain at any survival time, so the authors concluded the anterior lobe receives no direct innervation from brain neurons.

Results, 'The Innervation of the Anterior Lobe With AAV2/Retro or PRV 152 Injection'; Fig. 3

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SCH (Suprachiasmatic nucleus) UBERON:0002034 synapses onto IB (Interbrain) UBERON:0001894 · Rat

Neurons of the suprachiasmatic nucleus contained EGFP four days after posterior-lobe PRV 152 injection; since the virus spreads across synapses the connection may be mono- or polysynaptic.

Fig. 4A,B

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SFO (Subfornical organ) UBERON:0002219 synapses onto IB (Interbrain) UBERON:0001894 · Rat

PRV 152 placed in the posterior pituitary labelled cells in the subfornical organ at the 96 h survival point; the authors regard such regions as directly or indirectly connected to the posterior lobe.

Fig. 4A,B

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BLA (basal amygdaloid nucleus) UBERON:0002887 synapses onto IB (Interbrain) UBERON:0001894 · Rat

PRV 152 injected into the posterior lobe of the pituitary produced EGFP-labelled cell bodies in the posterior part of the basolateral amygdaloid nucleus at 96 h; the transneuronal nature of the tracer leaves open whether the link is direct or via intervening neurons.

Fig. 4A,B

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ARH (Arcuate hypothalamic nucleus) UBERON:0001932 synapses onto IB (Interbrain) UBERON:0001894 · Rat

Retrograde transneuronal labelling with PRV 152 from the posterior pituitary marked neurons in the arcuate hypothalamic nucleus four days after injection; direct versus multisynaptic connectivity cannot be separated with this method.

Fig. 4A,B

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root UBERON:0000955 projects to IB (Interbrain) UBERON:0001894 · Rat

A retrograde AAV2/Retro vector expressing EGFP was successfully delivered into the anterior lobe of the pituitary, yet no fluorescent cell bodies were found in any brain region or nucleus when the whole brain was surveyed, arguing against a direct neural projection to the anterior lobe.

Results, 'The Innervation of the Anterior Lobe With AAV2/Retro or PRV 152 Injection'

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

DMH (Dorsomedial nucleus of the hypothalamus) UBERON:0001934 synapses onto IB (Interbrain) UBERON:0001894 · Rat

EGFP-positive somata were detected in the dorsal portion of the dorsomedial hypothalamic nucleus 96 h after PRV 152 was injected into the posterior lobe, a labelling pattern the authors attribute to direct or indirect links with the posterior pituitary.

Fig. 4A,B

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.