Efferent and Afferent Connections of Neuropeptide Y Neurons in the Nucleus Accumbens of Mice

ACB (Nucleus accumbens) MBA:56 projects to PALv (Pallidum, ventral region) MBA:835 · Mouse

After Cre-dependent mCherry labelling of NAc NPY neurons, only a few labelled fibres were seen around the LH, including in the ventral pallidum.

Results, Visualization and Anterograde Tracing section

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

LPO (Lateral preoptic area) MBA:226 synapses onto ACB (Nucleus accumbens) MBA:56 · Mouse

A few presynaptic neurons to accumbal NPY cells, labelled by rabies tracing, were found in the lateral preoptic area.

Results, Retrograde Tracing of Global Inputs section

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PALv (Pallidum, ventral region) MBA:835 synapses onto ACB (Nucleus accumbens) MBA:56 · Mouse

The ventral pallidum contained a small number of rabies-labelled monosynaptic inputs to NAc NPY neurons.

Results, Retrograde Tracing of Global Inputs section

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ENT (Entorhinal area) MBA:909 synapses onto ACB (Nucleus accumbens) MBA:56 · Mouse

Few rabies-labelled presynaptic neurons to NAc NPY cells were seen in the entorhinal cortex.

Results, Retrograde Tracing of Global Inputs section

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLAp (Basolateral amygdalar nucleus, posterior part) MBA:311 synapses onto ACB (Nucleus accumbens) MBA:56 · Mouse

Monosynaptic rabies tracing found a minor input to NAc NPY neurons from the posterior basolateral amygdala.

Results, Retrograde Tracing of Global Inputs section

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PL (Prelimbic area) MBA:972 synapses onto ACB (Nucleus accumbens) MBA:56 · Mouse

A small number of rabies-labelled input neurons to NAc NPY cells were located in the prelimbic cortex.

Results, Retrograde Tracing of Global Inputs section

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

MTN (Midline group of the dorsal thalamus) MBA:571 synapses onto ACB (Nucleus accumbens) MBA:56 · Mouse

Monosynaptic rabies tracing from NAc NPY starter cells placed most of the presynaptic input neurons in the midline thalamic nuclei.

Fig. 6G

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

AI (Agranular insular area) MBA:95 synapses onto ACB (Nucleus accumbens) MBA:56 · Mouse

The insular cortex held a small number of monosynaptic inputs to accumbal NPY neurons labelled by rabies tracing.

Results, Retrograde Tracing of Global Inputs section

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) is unsure. “The paper says only 'insular cortex', so mapping it to the agranular insular area specifically cannot be confirmed.”

PF (Parafascicular nucleus) MBA:930 synapses onto ACB (Nucleus accumbens) MBA:56 · Mouse

A smaller number of monosynaptic rabies-labelled input neurons to NAc NPY neurons were found in the parafascicular thalamic nucleus.

Results, Retrograde Tracing of Global Inputs section

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BMAp (Basomedial amygdalar nucleus, posterior part) MBA:334 synapses onto ACB (Nucleus accumbens) MBA:56 · Mouse

The posterior basomedial amygdala was one of the two regions holding the vast majority of rabies-labelled inputs to NAc NPY neurons.

Fig. 6H

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACB (Nucleus accumbens) MBA:56 projects to LHA (Lateral hypothalamic area) MBA:194 · Mouse

Cre-dependent AAV-mCherry injected into the NAc of NPY-Cre mice labelled many punctate fibres in the anterior and posterior LH. A synaptophysin-EGFP AAV also produced EGFP presynaptic puncta in the LH, so the authors conclude that accumbal NPY neurons project mainly to the LH.

Figs. 2 and 3

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACB (Nucleus accumbens) MBA:56 projects to LHA (Lateral hypothalamic area) MBA:194 · Mouse

CTb injected into the LH retrogradely labelled ipsilateral NAc neurons, about 7% of them mCherry-labelled NPY neurons. In a second experiment, EnvA-pseudotyped rabies injected into the LH infected TVA-expressing NAc NPY neurons, labelling about 9.6% of the NPY neurons.

Fig. 4, Table 1; Fig. 5, Table 2

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) disagrees. “The 7.19% is the share of mCherry NPY neurons that were CTb-positive, not the share of CTb-labelled neurons that were NPY, so the paraphrase reverses the ratio.”