Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala

TH (Thalamus) MBA:549 projects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

Four weeks after AAV-ChR2-YFP injection into the posterior intralaminar and medial geniculate thalamic nuclei, YFP-labelled axons were dense in the lateral amygdala.

Representative Results, paragraph 2

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TH (Thalamus) MBA:549 projects to IA (Intercalated amygdalar nucleus) MBA:1105 · Mouse

Viral anterograde labelling from PIN/MGn produced dense ChR2-YFP axonal innervation of the medial paracapsular intercalated cell cluster.

Representative Results, paragraph 2

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TH (Thalamus) MBA:549 synapses onto Intercalated cell GABAergic neurons nt-y231zwbxhf · Mouse

Freeze-fracture replica immunolabelling showed ChR2-YFP-positive PIN/MGn terminals forming asymmetric synapses with AMPA- and NMDA-receptor-labelled postsynaptic specializations on spines and dendritic shafts of mu-opioid-receptor-positive ITC neurons; AMPA-R density was about 715 particles/um2 at spine synapses versus 590 at shaft synapses.

Figs. 5 and 6; Representative Results

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TH (Thalamus) MBA:549 projects to STR (Striatum) MBA:477 · Mouse

ChR2-YFP-expressing axons from the PIN/MGn injection site densely innervated the amygdalostriatal transition area.

Representative Results, paragraph 2

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.