Use of a tissue clearing technique combined with retrograde trans-synaptic viral tracing to evaluate changes in mouse retinorecipient brain regions following optic nerve crush

CL:0000740 synapses onto CEA (Central amygdalar nucleus) MBA:536 · Mouse

Unexpectedly, neurons in the central amygdala were labelled after intravitreal PRV724, predominantly contralateral to the injected eye, and contralateral labelling was strongly reduced after optic nerve crush.

Fig. 5A, Fig. 6A, C

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CL:0000740 synapses onto OP (Olivary pretectal nucleus) MBA:706 · Mouse

PRV724 labelling in the olivary pretectal nucleus was not reproducible across brains, so this target could not be assessed and was left out of the quantification.

Results, Overview of PRV724-traced retinorecipient regions

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CL:0000740 synapses onto GRN (Gigantocellular reticular nucleus) MBA:1048 · Mouse

PRV724 injected into the eye produced labelled neurons in the gigantocellular reticular nucleus bilaterally, with no change after optic nerve crush.

Fig. 5D, Fig. 7E, G

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CL:0000740 synapses onto LC (Locus ceruleus) MBA:147 · Mouse

Trans-synaptic PRV724 tracing from the retina labelled locus coeruleus neurons equally on both sides, and labelling was unaffected by optic nerve crush.

Fig. 5C, Fig. 7A, B

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CL:0000740 synapses onto IGL (Intergeniculate leaflet of the lateral geniculate complex) MBA:27 · Mouse

PRV724 injected into the eye labelled neurons in the intergeniculate leaflet of both hemispheres in light-sheet images of cleared brains; the contralateral count was reduced after optic nerve crush.

Fig. 5B, Fig. 6E, F

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CL:0000740 synapses onto LGv (Ventral part of the lateral geniculate complex) MBA:178 · Mouse

Trans-synaptic PRV724 from the eye produced labelled neurons in the ventral lateral geniculate nucleus bilaterally, and these numbers dropped in both hemispheres after optic nerve crush.

Fig. 5B, Fig. 6E, G

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CL:0000740 synapses onto RN (Red nucleus) MBA:214 · Mouse

Labelled neurons were unexpectedly found in the red nucleus of both hemispheres after intravitreal PRV724, and their number did not change after optic nerve crush.

Fig. 5C, Fig. 7A, D

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CL:0000740 synapses onto VII (Facial motor nucleus) MBA:661 · Mouse

Neurons of the facial motor nucleus were labelled bilaterally by intravitreal PRV724 in cleared brains, and counts were similar in control and optic nerve crush mice.

Fig. 5D, Fig. 7E, F

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CL:0000740 synapses onto SUV (Superior vestibular nucleus) MBA:217 · Mouse

PRV724 from the eye labelled superior vestibular nucleus neurons symmetrically in both hemispheres; counts were unchanged after optic nerve crush.

Fig. 5C, Fig. 7A, C

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CL:0000740 synapses onto BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

PRV724 trans-synaptic tracing from the retina labelled basolateral amygdala neurons equally in both hemispheres of cleared brains, and labelling decreased bilaterally after optic nerve crush.

Fig. 5A, Fig. 6A, B

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CL:0000740 synapses onto SCH (Suprachiasmatic nucleus) MBA:286 · Mouse

Although the suprachiasmatic nucleus was examined as a classical retinorecipient target, PRV724 labelling there was inconsistent between animals, so the authors excluded these data from analysis.

Results, Overview of PRV724-traced retinorecipient regions

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CL:0000740 synapses onto III (Oculomotor nucleus) MBA:35 · Mouse

Intravitreal PRV724 labelled oculomotor nucleus neurons, with more cells contralateral to the injected eye; contralateral labelling was reduced after optic nerve crush while ipsilateral labelling was unchanged.

Fig. 5B, Fig. 6E, H

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CL:0000740 synapses onto EW (Edinger-Westphal nucleus) MBA:975 · Mouse

Neurons in the midline Edinger-Westphal nucleus were labelled by intravitreally injected PRV724, and the number of labelled cells was reduced after optic nerve crush.

Fig. 5B, Fig. 6E, I

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CL:0000740 synapses onto SCs (Superior colliculus, sensory related) MBA:302 · Mouse

Labelling of superior colliculus neurons after intravitreal PRV724 was inconsistent between brains, and the authors therefore did not include superior colliculus data in their analysis.

Results, Overview of PRV724-traced retinorecipient regions

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CL:0000740 synapses onto PVH (Paraventricular hypothalamic nucleus) MBA:38 · Mouse

After intravitreal injection of the trans-synaptic pseudorabies virus PRV724, labelled neurons appeared in the paraventricular hypothalamic nucleus of cleared whole brains, with more labelling contralateral to the injected eye; labelling fell bilaterally after optic nerve crush.

Fig. 5A, Fig. 6A, D

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.