Dopamine neurons projecting to the posterior striatum form an anatomically distinct subclass

CL:0000700 projects to ORB (Orbital area) MBA:714 · Mouse

TVA injected into OFC led to rabies labelling of midbrain dopamine neurons, showing they project to OFC.

Figure 3—figure supplement 1, 2

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OT (Olfactory tubercle) MBA:754 synapses onto CL:0000700 · Mouse

VS-projecting dopamine neurons received relatively more rabies-labelled inputs from the olfactory tubercle.

Fig. 4

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CL:0000700 projects to PL (Prelimbic area) MBA:972 · Mouse

TVA injected into mPFC led to rabies labelling of midbrain dopamine neurons, showing they project to mPFC.

Figure 3—figure supplement 1, 2

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ACB (Nucleus accumbens) MBA:56 synapses onto CL:0000700 · Mouse

The ventral striatum supplied only a minor share of the rabies-labelled inputs to TS-projecting dopamine neurons. Those inputs were not concentrated in the ventral patches.

Fig. 4; Fig. 7A–D, I, J

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CL:0000700 projects to STRd (Striatum dorsal region) MBA:485 · Mouse

TVA delivered into the dorsal striatum, followed by rabies injected into VTA/SNc, labelled many midbrain neurons. Nearly all of these were TH-positive dopamine neurons projecting to DS.

Fig. 1B–F; Figure 3—figure supplement 1, 2

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Isocortex MBA:315 synapses onto CL:0000700 · Mouse

DS-projecting dopamine neurons received more monosynaptic inputs from neocortex than the other populations did.

Fig. 4

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STN (Subthalamic nucleus) MBA:470 synapses onto CL:0000700 · Mouse

TS-projecting dopamine neurons received many monosynaptic inputs from the subthalamic nucleus, significantly more than other populations (ANOVA p = 0.00023).

Fig. 3E; Fig. 4; Fig. 6D; Figure 4—figure supplement 1

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PSTN (Parasubthalamic nucleus) MBA:364 synapses onto CL:0000700 · Mouse

TS-projecting dopamine neurons received a large number of rabies-labelled inputs from the parasubthalamic nucleus.

Fig. 3E; Fig. 4; Fig. 6D

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GRN (Gigantocellular reticular nucleus) MBA:1048 synapses onto CL:0000700 · Mouse

The gigantocellular reticular nuclei supplied rabies-labelled inputs to the dopamine populations, with fewer going to lHb-projecting neurons.

Fig. 4

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LHA (Lateral hypothalamic area) MBA:194 synapses onto CL:0000700 · Mouse

Within the hypothalamus, the lateral hypothalamus (including PSTh) held the most monosynaptic inputs to dopamine neuron populations.

Fig. 4; Figure 4—figure supplement 1

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ACB (Nucleus accumbens) MBA:56 synapses onto CL:0000700 · Mouse

Monosynaptic rabies tracing showed the ventral striatum to be among the largest input sources for the canonical dopamine populations. In those populations, 12–20% of inputs lay in the ventral striatum, and 16–26% of accumbens inputs fell within five dense patches.

Fig. 3A; Fig. 4; Fig. 7D–J

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DR (Dorsal nucleus raphe) MBA:872 synapses onto CL:0000700 · Mouse

Every dopamine population tested received rabies-labelled inputs from the dorsal raphe.

Fig. 3H; Fig. 4

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CL:0000700 projects to GPe (Globus pallidus, external segment) MBA:1022 · Mouse

Cre-dependent TVA delivered into the globus pallidus retrogradely labelled a population of midbrain dopamine neurons after rabies injection into VTA/SNc.

Figure 3—figure supplement 1, 2

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CL:0000700 projects to LH (Lateral habenula) MBA:186 · Mouse

TVA injected into the lateral habenula labelled only a sparse population of midbrain dopamine neurons; 122 neurons in total were plotted.

Figure 3—figure supplement 2

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CL:0000700 projects to STRv (Striatum ventral region) MBA:493 · Mouse

After Cre-dependent TVA was delivered into the ventral striatum of DAT-Cre mice and EnvA rabies was injected into VTA/SNc, without RG, labelled dopamine neurons appeared in the midbrain, showing they project to VS.

Figure 3—figure supplement 1, 2; Figure 1—figure supplement 1A

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PAG (Periaqueductal gray) MBA:795 synapses onto CL:0000700 · Mouse

The periaqueductal grey supplied monosynaptic inputs to the dopamine populations, with fewer going to lHb-projecting neurons.

Fig. 4

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PALv (Pallidum, ventral region) MBA:835 synapses onto CL:0000700 · Mouse

The ventral pallidum was among the largest basal ganglia sources of rabies-labelled inputs to dopamine neuron populations.

Fig. 4

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CL:0000700 projects to CP (Caudoputamen) MBA:672 · Mouse

TVA injected into the posterior (tail) striatum labelled a dorsolaterally shifted population of midbrain dopamine neurons after rabies injection into VTA/SNc.

Figure 3—figure supplement 1, 2

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CL:0000700 projects to CEA (Central amygdalar nucleus) MBA:536 · Mouse

TVA delivered into the amygdala labelled dopamine neurons throughout VTA/SNc and also in the supramammillary A10rv area.

Results, Distribution of dopamine neurons; Figure 3—figure supplement 1

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STRd (Striatum dorsal region) MBA:485 synapses onto CL:0000700 · Mouse

The dorsal striatum was one of the largest basal ganglia sources of monosynaptic input to the dopamine populations. Its dorsal-most part contributed more to DS- and OFC-projecting neurons.

Fig. 4; Fig. 3B

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VMH (Ventromedial hypothalamic nucleus) MBA:693 synapses onto CL:0000700 · Mouse

The ventromedial hypothalamus was a somewhat larger input source to VS-projecting dopamine neurons; the difference was significant with TS excluded (ANOVA p = 0.0028).

Fig. 4; Figure 4—figure supplement 1

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ZI (Zona incerta) MBA:797 synapses onto CL:0000700 · Mouse

The zona incerta supplied a significantly larger share of inputs to TS-projecting dopamine neurons than to other populations (ANOVA p = 0.0028).

Fig. 3E; Fig. 4; Figure 4—figure supplement 1

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GPi (Globus pallidus, internal segment) MBA:1031 synapses onto CL:0000700 · Mouse

TS-projecting dopamine neurons received an increased proportion of monosynaptic inputs from the entopeduncular nucleus.

Fig. 3D; Fig. 4; Fig. 6C

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GPe (Globus pallidus, external segment) MBA:1022 synapses onto CL:0000700 · Mouse

A larger share of the inputs to TS-projecting dopamine neurons came from the globus pallidus than for other populations (ANOVA p = 0.0017).

Fig. 3C; Fig. 4; Fig. 6B; Figure 4—figure supplement 1

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