Nested circuits mediate the decision to vocalize

BST (Bed nuclei of the stria terminalis) MBA:351 synapses onto sAMY (Striatum-like amygdalar nuclei) MBA:278 · Mouse

Rabies tracing from AmgC/M-PAG neurons labeled presynaptic cells in the BNST.

Fig. 4C-D

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

LPO (Lateral preoptic area) MBA:226 synapses onto sAMY (Striatum-like amygdalar nuclei) MBA:278 · Mouse

Rabies tracing from AmgC/M-PAG neurons labeled presynaptic cells in the lateral preoptic area.

Fig. 4C-D

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

MPO (Medial preoptic area) MBA:523 projects to PAG (Periaqueductal gray) MBA:795 · Mouse

GFP-labeled POA neurons that project to AmgC/M also had axon terminals in the caudolateral PAG where PAG-USV neurons reside, indicating collateral projections.

Fig. 6C

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

MO (Somatomotor areas) MBA:500 synapses onto sAMY (Striatum-like amygdalar nuclei) MBA:278 · Mouse

Rabies tracing from AmgC/M-PAG starter neurons labeled input neurons in motor cortex.

Fig. 4B

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

MPO (Medial preoptic area) MBA:523 projects to sAMY (Striatum-like amygdalar nuclei) MBA:278 · Mouse

Cre-dependent GCaMP8s expressed in POA of Esr1-Cre males labeled axon terminals in the AmgC/M, from which terminal calcium activity was recorded.

Fig. 5A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) disagrees. “Fig. 5A is a viral schematic for fiber photometry of terminal calcium activity, not an anterograde tracing result.”

AUD (Auditory areas) MBA:247 synapses onto sAMY (Striatum-like amygdalar nuclei) MBA:278 · Mouse

Rabies tracing from AmgC/M-PAG starter neurons labeled input neurons in auditory cortex.

Fig. 4B

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

VTA (Ventral tegmental area) MBA:749 synapses onto sAMY (Striatum-like amygdalar nuclei) MBA:278 · Mouse

Rabies tracing from AmgC/M-PAG neurons labeled presynaptic cells in the VTA.

Fig. 4C-D

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACB (Nucleus accumbens) MBA:56 synapses onto sAMY (Striatum-like amygdalar nuclei) MBA:278 · Mouse

Rabies tracing from AmgC/M-PAG neurons labeled presynaptic cells in the nucleus accumbens shell.

Fig. 4C-D

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

sAMY (Striatum-like amygdalar nuclei) MBA:278 projects to PAG (Periaqueductal gray) MBA:795 · Mouse

Cre-dependent tdTomato injected into the amygdala of Esr1-Cre mice revealed labeled axons in the caudolateral PAG region containing PAG-USV neurons.

Fig. 3C

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PIR (Piriform area) MBA:961 synapses onto sAMY (Striatum-like amygdalar nuclei) MBA:278 · Mouse

Monosynaptic rabies tracing from PAG-projecting AmgC/M neurons in male mice labeled presynaptic cells in piriform cortex.

Fig. 4B

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

SS (Somatosensory areas) MBA:453 synapses onto sAMY (Striatum-like amygdalar nuclei) MBA:278 · Mouse

Rabies tracing from AmgC/M-PAG starter neurons labeled input neurons in somatosensory cortex.

Fig. 4B

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

AI (Agranular insular area) MBA:95 synapses onto sAMY (Striatum-like amygdalar nuclei) MBA:278 · Mouse

Rabies tracing from AmgC/M-PAG starter neurons labeled input neurons in insular cortex.

Fig. 4B

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) is unsure. “The paper says only insular cortex, so the mapping to the agranular insular subdivision specifically is not established.”

MPO (Medial preoptic area) MBA:523 projects to sAMY (Striatum-like amygdalar nuclei) MBA:278 · Mouse

AAVretro-Cre injected into the AmgC/M with Cre-dependent GFP in POA labeled POA cells projecting to the AmgC/M, most of which were Esr1-positive (298 of 362).

Fig. 6A-B

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

MPO (Medial preoptic area) MBA:523 functionally connects to sAMY (Striatum-like amygdalar nuclei) MBA:278 · Mouse

In slices, optogenetic activation of ChR2-expressing POAPAG axon terminals evoked monosynaptic IPSCs (in TTX/4AP) in most recorded AmgC/M-PAG neurons, and these were abolished by gabazine; no EPSCs were seen.

Fig. 6I-J

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

MPO (Medial preoptic area) MBA:523 synapses onto sAMY (Striatum-like amygdalar nuclei) MBA:278 · Mouse

Monosynaptic rabies tracing from AmgC/M-PAG neurons labeled cell bodies in the medial preoptic area.

Fig. 4C-E

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

sAMY (Striatum-like amygdalar nuclei) MBA:278 projects to PAG (Periaqueductal gray) MBA:795 · Mouse

Injecting AAVretro-Cre into caudolateral PAG plus Cre-dependent virus in the amygdala labeled PAG-projecting neurons in the AmgC/M but not the CeA of female mice; these neurons were all VGAT-positive.

Fig. 2B; Fig. 2E

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.