clm-h7ehw3ksx4

PL (Prelimbic area) functionally connects to DR (Dorsal nucleus raphe)

Species: Rat
Result: present
Method: optogenetic circuit mapping
Sign: excitatory
Evidence: With ChR2 expressed in mPFC and DRN neurons retrogradely labelled from mPFC, light stimulation of mPFC axons within DRN evoked excitatory postsynaptic currents in mPFC-projecting 5-HT neurons (recorded with picrotoxin present); these currents were abolished by TTX and restored by 4-AP, indicating a direct monosynaptic glutamatergic input. Amplitudes averaged about 47 pA in controls and were larger in prenatally ethanol-exposed rats, which also showed a reduced paired-pulse ratio; the NO donor SNAP potentiated these currents in controls.
Source: Chemogenetic inhibition of prefrontal cortex inputs to dorsal raphe reduces anxiety behaviors in male rat model of fetal alcohol spectrum disorder
Scientific Reports, 2025
DOI 10.1038/s41598-025-99181-8 · PubMed 40275074 · PMC12022358, Fig. 2A–D; Fig. 3D–E
Licence: CC-BY-NC-ND-4.0
Named in the paper: “mPFC” and “DRNmPFC 5-HT neurons”
Made by: an AI model reading the paper (claude-opus-5, extract@0.3.0), 2026-10-09
Checked by: a second AI model (claude-opus-5) agrees, 2026-10-09
“Monosynaptic mPFC glutamatergic input to DRNmPFC 5-HT neurons, potentiated by PE and by SNAP in controls.”
Status: proposed
Drafted and checked by AI models; it stays proposed until people have audited a sample of such claims.