Rat
PL (Prelimbic area) functionally connects to DR (Dorsal nucleus raphe)
2 claims from 1 source: 2 found, 0 tested and absent, 0 ambiguous. Evidence: optogenetic circuit mapping.
The claims behind it
With ChR2 expressed in mPFC and DRN neurons retrogradely labelled from mPFC, light stimulation of mPFC axons within DRN evoked excitatory postsynaptic currents in mPFC-projecting 5-HT neurons (recorded with picrotoxin present); these currents were abolished by TTX and restored by 4-AP, indicating a direct monosynaptic glutamatergic input. Amplitudes averaged about 47 pA in controls and were larger in prenatally ethanol-exposed rats, which also showed a reduced paired-pulse ratio; the NO donor SNAP potentiated these currents in controls.
DOI 10.1038/s41598-025-99181-8 · PubMed 40275074 · PMC12022358, Fig. 2A–D; Fig. 3D–EMade by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.
In slices with ChR2 in mPFC and DRN neurons retrogradely labelled from the central amygdala, photostimulation of mPFC fibres evoked monosynaptic excitatory currents (TTX-sensitive, rescued by 4-AP) in CeA-projecting 5-HT neurons, with mean amplitude near 49 pA in both control and prenatal-ethanol rats; paired-pulse ratio was lower after prenatal ethanol and SNAP enhanced the currents in controls.
DOI 10.1038/s41598-025-99181-8 · PubMed 40275074 · PMC12022358, Fig. 5A–D; Fig. 6D–EMade by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.