Mouse
BLAa (Basolateral amygdalar nucleus, anterior part) functionally connects to PL (Prelimbic area)
4 claims from 1 source: 3 found, 1 tested and absent, 0 ambiguous. Evidence: optogenetic circuit mapping.
The claims behind it
In awake mice, optical stimulation of rBLA with 20 Hz trains changed the firing of a substantial share of single units recorded with silicon probes in PL: about a fifth of units in layer 2 and a fifth in layer 5 responded, with activation dominating in layer 2 and suppression dominating in layer 5.
DOI 10.7554/elife.82688 · PubMed 36476757 · PMC9803354, Fig. 4C,E,F; Fig. 4—figure supplements 1 and 2Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.
When rBLA fibres were stimulated at intensities that made PL L2 CA neurons fire, PL L5 PT neurons showed large excitatory (-113 ± 24 pA) and inhibitory (508 ± 101 pA) currents, whereas subthreshold stimulation produced almost nothing (-9 ± 4 pA and 17 ± 5 pA; 8 pairs, 4 animals), indicating an indirect route through superficial layers.
DOI 10.7554/elife.82688 · PubMed 36476757 · PMC9803354, Fig. 5A–CMade by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.
Light activation of ChR2-expressing rBLA axons in slices evoked monosynaptic glutamatergic currents (isolated with TTX, 4-AP and raised Ca2+) that were far larger in PL layer 2 cortico-amygdalar neurons than in any other recorded projection neuron, and sCRACM showed the input lands on basal and perisomatic dendrites. In current clamp these inputs reliably drove action potentials (firing probability 0.88 ± 0.05, 8 pairs). Responses were also larger at PL L2 CA cells than neighbouring L2 cortico-cortical cells.
DOI 10.7554/elife.82688 · PubMed 36476757 · PMC9803354, Fig. 2C, 2F; Fig. 3A,B,E; Fig. 2—figure supplement 1A–CMade by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.
Subcellular mapping of rBLA axons under TTX/4-AP revealed no monosynaptic input to either the apical or the basal dendrites of PL layer 5 pyramidal tract neurons, and subthreshold stimulation of rBLA fibres evoked essentially no current in these cells; the authors conclude there is no direct rBLA to PL L5 PT connection.
DOI 10.7554/elife.82688 · PubMed 36476757 · PMC9803354, Fig. 2F; Fig. 2—figure supplement 1I; Fig. 5A–CMade by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.