Mouse
BST (Bed nuclei of the stria terminalis) functionally connects to LHA (Lateral hypothalamic area)
3 claims from 2 sources: 3 found, 0 tested and absent, 0 ambiguous. Evidence: optogenetic circuit mapping.
The claims behind it
Photostimulating ChR2-expressing BST VGAT axons in LH slices evoked fast postsynaptic currents in identified MCH cells that reversed near the chloride equilibrium potential, consistent with direct GABAergic inhibition; mean amplitude about 118 pA.
DOI 10.1038/ncomms11395 · PubMed 27102565 · PMC4844703, Fig. 6c,eMade by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.
A minority of the synaptically connected LHA neurons (6 of 42) responded to optical stimulation of BNST axons with light-evoked EPSCs only; in the rare cells where evoked EPSCs persisted with TTX, 4-AP and gabazine, the AMPA-receptor blocker DNQX confirmed glutamatergic transmission.
DOI 10.1016/j.molmet.2023.101702 · PubMed 36898526 · PMC10025284, Fig. 3F, Fig. S3; Methods (voltage clamp)Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.
After ChR2 was expressed in BNST neurons, blue light stimulation of BNST axons in LHA slices evoked postsynaptic currents in 42 of 70 patched LHA neurons; 36 of those 42 connected cells showed light-evoked IPSCs that persisted with TTX/4-AP (monosynaptic) and were nearly abolished by the GABA-A antagonist gabazine. Connected cells were spread along the anterior–posterior LHA but were most common in middle and posterior LHA.
DOI 10.1016/j.molmet.2023.101702 · PubMed 36898526 · PMC10025284, Fig. 3E–I, Figs. S2E–GMade by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.