mGluR5 in EC CCK to BLA Circuit Modulates Depressive‐Like Phenotypes through CCK Signaling

ENT (Entorhinal area) MBA:909 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Cre-dependent EYFP injected into the entorhinal cortex of CCK-Cre mice produced anterogradely labelled axonal fibres within the BLA, confirming the ECCCK to BLA projection.

Fig. 1C,D; Results, first section

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

ENT (Entorhinal area) MBA:909 functionally connects to BLA glutamatergic principal neurons nt-wprma5fcrr · Mouse

Optogenetic activation of ChrimsonR-expressing entorhinal CCK neurons in CCK-Cre mice rapidly increased GCaMP6s calcium signals in CaMKII-positive BLA neurons, and the same stimulation elevated a BLA CCK sensor signal; the calcium response was strongly reduced after the mGluR5 agonist DHPG.

Fig. 1E–I; Fig. 5E–J, M–O

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

ENT (Entorhinal area) MBA:909 synapses onto BLA glutamatergic principal neurons nt-wprma5fcrr · Mouse

Anterogradely transported AAV1-hSyn-Cre injected in the entorhinal cortex combined with a Cre-dependent CaMKII-driven reporter in the BLA labelled BLA glutamatergic neurons receiving entorhinal input; expansion microscopy showed these postsynaptic sites carried mGluR5 colocalized with PSD-95.

Fig. 3E–I; Results, mGluR5 enrichment section

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

ENT (Entorhinal area) MBA:909 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

A retrograde AAV carrying a Cre-dependent EYFP was placed in the BLA of CCK-Cre mice; five weeks later, retrogradely labelled CCK-expressing cell bodies appeared in the entorhinal cortex, indicating an entorhinal CCK projection reaching the BLA.

Fig. 1A,B; Results, first section

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.