The anterior insula and its projection to amygdala nuclei modulate the abstinence-exacerbated expression of conditioned place preference

AI (Agranular insular area) MBA:95 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

After bilateral injection of AAV5-CaMKIIa-eYFP (or eNpHR3.0-eYFP) into the anterior insula, eYFP-labelled axon terminals were found in the basolateral amygdala. Quantification of nine confocal images from a representative animal showed the mean eYFP intensity was significantly greater in BLA than in CeA (paired t-test, t(8) = 8.04, p < 0.001).

Fig. 3b, c; Results, aIC-AMY photoinhibition section

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

AI (Agranular insular area) MBA:95 projects to CEA (Central amygdalar nucleus) MBA:536 · Mouse

eYFP-positive axons arising from CaMKIIa-expressing anterior insular neurons were also detected in the central amygdala, although the mean fluorescence intensity of these terminals was significantly lower than that measured in the basolateral amygdala.

Fig. 3b, c; Results, aIC-AMY photoinhibition section

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.