Amygdala GABA Neurons Project To vlPAG And mPFC

CEA (Central amygdalar nucleus) MBA:536 projects to PAG (Periaqueductal gray) MBA:795 · Mouse

A Cre-dependent retrograde AAV expressing tdTomato was injected into the vlPAG of VGAT-Cre mice, and it labelled many GABAergic neurons in the central amygdala. About 85% of retrogradely labelled CeA neurons were tdTomato+, and about 15% of all labelled CeA neurons were double-labelled, meaning they also projected to the mPFC.

Results; Fig. 3A-C; Table 1

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 projects to Isocortex MBA:315 · Mouse

After GFP retrograde AAV was injected into the mPFC, BLA neurons were labelled. About 43% of labelled BLA cells projected only to the mPFC, and about 28% were bifurcating GABAergic cells that projected to both the mPFC and the vlPAG.

Results; Fig. 3F; Fig. 4B-C; Table 1

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

CEA (Central amygdalar nucleus) MBA:536 projects to Isocortex MBA:315 · Mouse

A retrograde AAV expressing GFP from a generic promoter was injected into the mPFC, and it labelled CeA neurons. About 15% of labelled CeA cells projected only to the mPFC, and a further ~15% were double-labelled cells that also projected to the vlPAG.

Results; Fig. 3C; Fig. 4A; Table 1

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 projects to PAG (Periaqueductal gray) MBA:795 · Mouse

After a Cre-dependent retrograde tdTomato AAV was injected into the vlPAG of VGAT-Cre mice, GABAergic neurons in the basolateral amygdala were labelled. About 57% of labelled BLA neurons projected to the vlPAG, and roughly 28% were bifurcating cells that also projected to the mPFC.

Results; Fig. 3D-F; Fig. 4B-C; Table 1

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.