Effects of chronic ethanol exposure on dorsal medial striatal neurons receiving convergent inputs from the orbitofrontal cortex and basolateral amygdala

ORB (Orbital area) MBA:714 synapses onto CP (Caudoputamen) MBA:672 · Mouse

An anterograde transsynaptic AAV1 carrying Cre was placed in the orbitofrontal cortex together with an AAV1-Flpo in the basolateral amygdala and a Cre-dependent/Flp-dependent EYFP reporter in the dorsomedial striatum. EYFP-labelled striatal neurons appeared only when both recombinase viruses were delivered, indicating that orbitofrontal axons transsynaptically label dorsomedial striatal cells, which were then recorded in slices.

Fig. 1A–B; Results, first paragraph

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 synapses onto CP (Caudoputamen) MBA:672 · Mouse

AAV1-Flpo injected into the basolateral amygdala, combined with AAV1-Cre in the orbitofrontal cortex and a double-dependent EYFP reporter in the dorsomedial striatum, produced EYFP-positive striatal neurons; no reporter signal was seen when only one recombinase virus was injected, showing that amygdalar axons transsynaptically label dorsomedial striatal neurons that also receive cortical input.

Fig. 1A–B; Results, first paragraph

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.