Genetic identification of a neural circuit that suppresses appetite

PB (Parabrachial nucleus) MBA:867 projects to CEAc (Central amygdalar nucleus, capsular part) MBA:544 · Mouse

Cre-dependent mCherry and synaptophysin-GFP viruses injected into the PBN of CalcaCre mice labelled abundant axons and presynaptic terminals in the laterocapsular central amygdala, overlapping with CGRP immunostaining of terminals there.

Fig. 4a

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PB (Parabrachial nucleus) MBA:867 functionally connects to CEAc (Central amygdalar nucleus, capsular part) MBA:544 · Mouse

In slices, light activation of ChR2-expressing PBelo CGRP axons in the CeAlc evoked excitatory postsynaptic currents and raised firing in CeAlc neurons; both responses were abolished by CNQX and APV, indicating glutamatergic transmission. In vivo photostimulation of either the PBelo cell bodies or their CeAlc terminals increased Fos in the CeAlc.

Fig. 4b; Supplementary Fig. 14

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PB (Parabrachial nucleus) MBA:867 projects to MED (Medial group of the dorsal thalamus) MBA:444 · Mouse

Sparse mCherry and synaptophysin-GFP labelling from PBelo CGRP neurons was found in the medial thalamus.

Results (projection mapping)

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PB (Parabrachial nucleus) MBA:867 projects to PVH (Paraventricular hypothalamic nucleus) MBA:38 · Mouse

Labelled fibers and terminals from PBelo CGRP neurons were not observed in the paraventricular hypothalamic nucleus.

Results (projection mapping)

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PB (Parabrachial nucleus) MBA:867 projects to BST (Bed nuclei of the stria terminalis) MBA:351 · Mouse

Cre-dependent mCherry and synaptophysin-GFP expressed in PBelo CGRP neurons labelled fibers and terminals in the BNST, though less abundantly than in the CeAlc.

Results (projection mapping); Supplementary Fig. 12

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PB (Parabrachial nucleus) MBA:867 projects to ARH (Arcuate hypothalamic nucleus) MBA:223 · Mouse

No mCherry or synaptophysin-GFP labelling from PBelo CGRP neurons was detected in the arcuate nucleus.

Results (projection mapping)

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PB (Parabrachial nucleus) MBA:867 projects to CEAc (Central amygdalar nucleus, capsular part) MBA:544 · Mouse

Green retrobeads placed in the CeAlc retrogradely labelled neurons in the PBelo, and over 95% of these retrogradely labelled cells also expressed the Cre-dependent mCherry marking CGRP neurons. Retrograde CAV2-Cre injected into the CeAlc likewise drove Cre-dependent transgene expression in upstream PBN neurons.

Results (projection mapping); Supplementary Fig. 13; Fig. 4e

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

PB (Parabrachial nucleus) MBA:867 projects to LHA (Lateral hypothalamic area) MBA:194 · Mouse

Only sparse mCherry- and synaptophysin-GFP-labelled fibers from PBelo CGRP neurons were seen in the lateral hypothalamus.

Results (projection mapping)

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.