Hyperconnectivity of prefrontal cortex to amygdala projections in a mouse model of macrocephaly/autism syndrome

PL (Prelimbic area) MBA:972 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Retrograde CAV2-Cre was injected into the BLA and Cre-dependent mCherry AAV into the mPFC. This labelled BLA-projecting neurons, which the figure shows in the prelimbic cortex.

Fig. 6a,d,e

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

Isocortex MBA:315 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

An AAV expressing tdTomato was injected into the mPFC of adult female mice, and labelled axon terminals with branches and synaptic boutons were traced in the BLA. Branching and bouton density were higher in Pten+/− than in wild-type mice.

Fig. 3a–e; Fig. 5c–f

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) disagrees. “The AAV-tdTomato tracing supports mPFC to BLA, but the subject is mapped to the whole isocortex, which is broader than the mPFC the paper means.”

ILA (Infralimbic area) MBA:44 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Intersectional labelling with CAV2-Cre in the BLA and Cre-dependent mCherry in the mPFC revealed BLA-projecting neurons, shown in the infralimbic cortex.

Fig. 6a,d,e

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.