Dopamine release and dopamine-related gene expression in the amygdala are modulated by the gastrin-releasing peptide in opposite directions during stress-enhanced fear learning and extinction

HIP (Hippocampal region) MBA:1080 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Retrograde rAAV2 injected into the basal amygdala labelled ventral hippocampal neurons, which overlapped with GRP-positive cells.

Fig. 1H, 1J

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

MGm (Medial geniculate complex, medial part) MBA:1088 projects to AUD (Auditory areas) MBA:247 · Mouse

After rAAV2-retro-CaMKII-tdTomato injection into auditory cortex area TE3, retrogradely labelled cells, some of them GRP-positive, appeared in the MGm/PIN region of the auditory thalamus.

Fig. 1F, 1J

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

VTA (Ventral tegmental area) MBA:749 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

AAV-hSyn-ChR2-EYFP was injected into the VTA, with the injection site including TH-positive cells. GFP-immunolabelled axons were found in the basal amygdala, where they overlapped with TH staining.

Fig. 5A, 5B

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

AUD (Auditory areas) MBA:247 projects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

Retrograde rAAV2 tracer injected into the LA labelled neurons in auditory cortex area TE3, including GRP-positive cells.

Fig. 1G, 1J

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

MGm (Medial geniculate complex, medial part) MBA:1088 projects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

An LA injection of retrograde rAAV2 labelled cells in the MGm/PIN thalamic area. None of these labelled cells were GRP-positive.

Supplementary Fig. 2

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

VTA (Ventral tegmental area) MBA:749 functionally connects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

In slices from wildtype mice, light stimulation of ChR2-expressing VTA terminals raised the frequency of spontaneous EPSPs in BLA neurons. It did not change sEPSP amplitude or sIPSP frequency or amplitude. In Grp-/- mice, baseline sEPSP frequency was already high and light did not change it further.

Fig. 5C-J

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 projects to Isocortex MBA:315 · Mouse

After retrograde rAAV2 injection into the mPFC, most amygdala labelling was in the GRP-negative basal nucleus. No labelled amygdala cells were GRP-positive.

Fig. 1I

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.