The parabrachial to central amygdala pathway is critical to injury-induced pain sensitization in mice

PB (Parabrachial nucleus) MBA:867 functionally connects to CEAl somatostatin-expressing neurons nt-t6ymz01hv4 · Mouse

Optogenetic activation of PBN axon terminals in CeA slices from Sst-cre::Ai9 mice produced light-evoked EPSCs in nearly all recorded somatostatin-positive laterocapsular CeA neurons, independent of their firing type.

Fig. 1C–F; Results, section 1

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PB (Parabrachial nucleus) MBA:867 projects to CEA (Central amygdalar nucleus) MBA:536 · Mouse

After injecting an AAV expressing ChR2-EYFP into the PBN, fluorescent PBN axon terminals were seen in the laterocapsular part of the central amygdala.

Fig. 1B; Results, section 1

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PB (Parabrachial nucleus) MBA:867 projects to CEA (Central amygdalar nucleus) MBA:536 · Mouse

A retrograde Cre-expressing AAV injected into the CeA, combined with a Cre-dependent mCherry AAV in the PBN, labelled CeA-projecting neurons across the rostrocaudal PBN, mostly confined to the external lateral subdivision. Retrobeads injected into the CeA also labelled PBN neurons that were then recorded.

Fig. S1E–G; Fig. 2F

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PB (Parabrachial nucleus) MBA:867 functionally connects to CEAl PKC-delta-expressing neurons nt-g513b58b0y · Mouse

Blue-light stimulation of ChR2-expressing PBN terminals in CeA slices from Prkcd-cre::Ai9 mice evoked optically evoked EPSCs in most recorded PKCδ+ neurons in the laterocapsular CeA, regardless of whether the cells were late-firing or regular-spiking.

Fig. 1C–F; Results, section 1

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.