Input associativity underlies fear memory renewal

AUD (Auditory areas) MBA:247 functionally connects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

Electrical stimulation of ACx evoked corticoamygdalar transmission in LA neurons. The transmission was reduced by yellow-light inhibition of eNpHR-expressing ACx terminals.

Supplementary Fig. 9

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AUD (Auditory areas) MBA:247 projects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

Viral expression of ChR2-mCherry or eNpHR-EYFP in ACx labelled axonal terminals in the LA.

Fig. 3J; Fig. 4E

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AUD (Auditory areas) MBA:247 synapses onto LA (Lateral amygdalar nucleus) MBA:131 · Mouse

AAV1-Cre anterograde transsynaptic tracing from ACx in IS reporter mice labelled postsynaptic neurons, with the LA as the preferential target.

Supplementary Fig. 6B–D

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AUD (Auditory areas) MBA:247 synapses onto BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Anterograde AAV1-Cre transsynaptic labelling from ACx reached basal amygdala neurons at a moderate level.

Supplementary Fig. 6B–D

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AUD (Auditory areas) MBA:247 functionally connects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

Optogenetic stimulation of ACx fibres in the LA evoked monosynaptic AMPAR/NMDAR-mediated EPSCs in LA neurons. In the same LA neurons, the dual-colour experiments also evoked feedforward IPSCs.

Fig. 3I–P; Supplementary Fig. 7D–F; Fig. 5C–D

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HIP (Hippocampal region) MBA:1080 synapses onto BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

AAV1-Cre anterograde transsynaptic tracing from vHPC labelled a low proportion of basal amygdala neurons.

Fig. 2H–L

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SS (Somatosensory areas) MBA:453 functionally connects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

Optogenetic stimulation of SCx inputs evoked synaptic responses in LA neurons, some of which also received ACx input. Paired-pulse ratios did not change with fear renewal.

Supplementary Fig. 8A–M

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AUD (Auditory areas) MBA:247 synapses onto CEA (Central amygdalar nucleus) MBA:536 · Mouse

Anterograde AAV1-Cre transsynaptic labelling from ACx produced only minimal labelling in the central amygdala.

Supplementary Fig. 6B–D

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HIP (Hippocampal region) MBA:1080 synapses onto LA (Lateral amygdalar nucleus) MBA:131 · Mouse

AAV1-Cre from vHPC labelled a small proportion of LA neurons as postsynaptic targets. Among these vHPC recipients, about 8% also received ACx input.

Fig. 2H–L

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HIP (Hippocampal region) MBA:1080 synapses onto CEA (Central amygdalar nucleus) MBA:536 · Mouse

AAV1-Cre anterograde transsynaptic tracing from vHPC labelled a low proportion of central amygdala neurons. None of these labelled neurons received convergent ACx input.

Fig. 2H–L

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HIP (Hippocampal region) MBA:1080 projects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

After retrograde AAV injection into the LA, far fewer labelled neurons were seen in the dorsal hippocampus than in the ventral hippocampus.

Supplementary Fig. 5

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HIP (Hippocampal region) MBA:1080 functionally connects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

Photostimulating ChR2-expressing vHPC terminals evoked AMPAR- and NMDAR-mediated EPSCs in every recorded LA neuron. The responses were abolished by TTX and restored by 4-AP, which indicates monosynaptic input. Feedforward IPSCs were also evoked.

Fig. 3A–H; Supplementary Fig. 7A–C; Fig. 5C–F

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HIP (Hippocampal region) MBA:1080 projects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

Injecting retrograde AAV (rAAV2-retro-Syn-mCherry) into the LA labelled many LA-projecting neurons in the ventral hippocampus.

Fig. 2A–C

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HIP (Hippocampal region) MBA:1080 projects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

After AAV-CaMKIIα-ChR2-mCherry was injected into vHPC, mCherry-labelled terminals appeared in the amygdala, including the LA.

Fig. 3B

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AUD (Auditory areas) MBA:247 projects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

Retrograde AAV injected into the LA also labelled LA-projecting neurons in the auditory cortex.

Fig. 2D–F

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