The medial orbitofrontal cortex mediates neuropathic pain and anxiodepressive-like behaviors via two distinct pathways

ORBm (Orbital area, medial part) MBA:731 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Anterograde mGFP/synaptophysin-mRuby labelling from CaMKIIα+ mOFC neurons showed axonal fibers with presynaptic puncta in the basolateral amygdala.

Fig. 4A, B, D

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ORBm (Orbital area, medial part) MBA:731 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Retrograde AAV injected into the BLA labelled mOFC neurons, including CaMKIIα+ cells, that did not overlap with MD-projecting mOFC neurons.

Fig. 4E-I; Fig. S4

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ORBm (Orbital area, medial part) MBA:731 projects to MD (Mediodorsal nucleus of thalamus) MBA:362 · Mouse

Cre-dependent mGFP and synaptophysin-mRuby expressed in CaMKIIα+ mOFC neurons of CaMKII-Cre mice labelled axons with colocalized presynaptic puncta in the mediodorsal thalamus.

Fig. 4A-C

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ORBm (Orbital area, medial part) MBA:731 projects to MD (Mediodorsal nucleus of thalamus) MBA:362 · Mouse

Retrograde AAV (hSyn in C57 mice, Cre-dependent in CaMKII-Cre mice) injected into MD labelled neurons in the mOFC, a population largely distinct from BLA-projecting cells.

Fig. 4E-I; Fig. S4

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ORBm (Orbital area, medial part) MBA:731 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

Whole-brain sections after anterograde labelling of CaMKIIα+ mOFC neurons showed labelled projections in the anterior cingulate cortex among other targets.

Fig. S3

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ORBm (Orbital area, medial part) MBA:731 projects to ACB (Nucleus accumbens) MBA:56 · Mouse

Anterograde labelling from CaMKIIα+ mOFC neurons revealed projections reaching the nucleus accumbens.

Fig. S3

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.