Memory retrieval in addiction: a role for miR-105-mediated regulation of D1 receptors in mPFC neurons projecting to the basolateral amygdala

HPF (Hippocampal formation) UBERON:0002421 projects to BLA (basal amygdaloid nucleus) UBERON:0002887 · Rat

BDA injected into the hippocampus produced labelled axon terminals in the BLA that co-localised with VGLUT2, showing a glutamatergic hippocampal input to the BLA; the density of this BDA/VGLUT2 labelling was unchanged by morphine treatment.

Fig. 2a; Methods, Anterograde tracing

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PL (Prelimbic area) MBA:972 functionally connects to BLA glutamatergic principal neurons nt-wprma5fcrr · Mouse

Blue-light stimulation of ChR2-expressing mPFC axons in BLA slices evoked EPSCs in BLA pyramidal neurons (about 100-200 pA, mean ~170-180 pA) that were abolished by the AMPA receptor antagonist DNQX, identifying a glutamatergic mPFC-to-BLA synaptic input; paired-pulse responses and AMPA/NMDA ratios were also measured at this input.

Fig. 7a (right panel), Fig. 7b,c; Fig. 8

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PL (Prelimbic area) MBA:972 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Six weeks after injecting AAV-CaMKIIa-ChR2-mCherry into the mPFC, mCherry-labelled axons from the mPFC were seen in the BLA, described by the authors as strong fibre labelling.

Fig. 7a (left and middle panels)

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PL (Prelimbic area) UBERON:8440032 projects to BLA (basal amygdaloid nucleus) UBERON:0002887 · Rat

Fluorescent microspheres and, in a separate experiment, Fluorogold injected into the BLA retrogradely labelled neurons in the mPFC, which were then sampled for single-cell RT-PCR or stained for c-Fos, confirming mPFC neurons that project to the BLA.

Fig. 6a; Fig. 9b; Methods, Single-cell RT-PCR and c-Fos double immunofluorescence

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PL (Prelimbic area) UBERON:8440032 projects to BLA (basal amygdaloid nucleus) UBERON:0002887 · Rat

BDA injected into the medial prefrontal cortex labelled axon terminals in the basolateral amygdala, where BDA-positive puncta co-localised with the glutamatergic terminal marker VGLUT2, indicating a glutamatergic mPFC projection to the BLA.

Fig. 1a,b; Methods, Anterograde tracing

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TH (Thalamus) UBERON:0001897 projects to BLA (basal amygdaloid nucleus) UBERON:0002887 · Rat

BDA injected into the thalamus labelled axon terminals in the BLA that co-localised with VGLUT2, demonstrating a glutamatergic thalamic projection to the BLA.

Fig. 2b; Methods, Anterograde tracing

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