The amygdala modulates prepulse inhibition of the auditory startle reflex through excitatory inputs to the caudal pontine reticular nucleus

BST (Bed nuclei of the stria terminalis) MBA:351 projects to PRNc (Pontine reticular nucleus, caudal part) MBA:1093 · Mouse

Retrograde Fluoro-Gold from the PnC labelled neurons in the intra-amygdaloid division of the BNST.

Results, section 1

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CEA (Central amygdalar nucleus) MBA:536 functionally connects to PRNc (Pontine reticular nucleus, caudal part) MBA:1093 · Mouse

In PnC slices from GlyT2-Cre mice, blue-light stimulation of ChR2-expressing CeA fibres evoked facilitating EPSPs and EPSCs in tdTomato-labelled GlyT2+ neurons. AP5 and DNQX reduced these responses, and no light-evoked IPSCs appeared. Responsive cells lay medial to the 7th nerve or lateroventrally; midline and contralateral cells did not respond.

Fig. 8-9

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CP (Caudoputamen) MBA:672 projects to PRNc (Pontine reticular nucleus, caudal part) MBA:1093 · Mouse

Fluoro-Gold injected into the PnC retrogradely labelled neurons in the caudate putamen.

Results, section 1

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PPN (Pedunculopontine nucleus) MBA:1052 projects to PRNc (Pontine reticular nucleus, caudal part) MBA:1093 · Mouse

Fluoro-Gold injected into the PnC retrogradely labelled neurons in the PPTg in four mice.

Additional file 1: Figure S1

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CEA (Central amygdalar nucleus) MBA:536 projects to PRNc (Pontine reticular nucleus, caudal part) MBA:1093 · Mouse

AAV-CamKIIα-eYFP injected into the CeA labelled largely VGLUT2+ neurons. Their axons coursed mainly ipsilaterally through the lateroventral PnC. In GlyT2-eGFP mice, CamKIIα-mCherry CeA fibres formed close appositions, many PSD-95-positive, onto GlyT2+ PnC neurons.

Fig. 3f; Fig. 7

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CEA (Central amygdalar nucleus) MBA:536 projects to PRNc (Pontine reticular nucleus, caudal part) MBA:1093 · Mouse

A unilateral Fluoro-Gold injection confined to the PnC retrogradely labelled CeA cell bodies ipsilateral to the injection. The labelled cells clustered near the dorsomedial border of the amygdalar complex.

Fig. 2e-f

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LHA (Lateral hypothalamic area) MBA:194 projects to PRNc (Pontine reticular nucleus, caudal part) MBA:1093 · Mouse

PnC Fluoro-Gold injections labelled neurons in the lateral hypothalamus.

Results, section 1

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.