Peripheral nerve injury reduces the excitation-inhibition balance of basolateral amygdala inputs to prelimbic pyramidal neurons projecting to the periaqueductal gray

PL (Prelimbic area) MBA:972 projects to PAG (Periaqueductal gray) MBA:795 · Mouse

Alexa 647-conjugated cholera toxin B injected into the PAG retrogradely labeled cortico-PAG neurons in the PL cortex, which were then targeted for recording.

Fig. 1a, d, e

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 projects to PL (Prelimbic area) MBA:972 · Mouse

After AAV-ChR2-Venus injection into the BLA, Venus-labeled BLA axons were seen in PL cortex overlapping retrogradely labeled cortico-PAG neurons.

Fig. 1d

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 functionally connects to PL5 (Prelimbic area, layer 5) MBA:363 · Mouse

Blue-light stimulation of ChR2-expressing BLA axons in slices evoked both EPSCs (at -70 mV) and IPSCs (at +10 mV) in PL layer 5 cortico-PAG neurons; the E/I ratio was reduced after spared nerve injury.

Fig. 1f-h, m, n

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 projects to ILA (Infralimbic area) MBA:44 · Mouse

ChR2-Venus-expressing BLA axons were observed in IL cortex, overlapping retrogradely labeled cortico-PAG somata.

Fig. 1d

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 functionally connects to ILA5 (Infralimbic area, layer 5) MBA:827 · Mouse

Optogenetic activation of BLA axons evoked excitatory and inhibitory postsynaptic currents in IL layer 5 cortico-PAG neurons, with E/I ratio unchanged by nerve injury.

Fig. 1j-l, m, n

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ILA (Infralimbic area) MBA:44 projects to PAG (Periaqueductal gray) MBA:795 · Mouse

CTB injected into the PAG produced retrogradely labeled cortico-PAG somata in IL cortex, including layer 5 cells used for recordings.

Fig. 1a, d, i

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.