Roles of mediodorsal thalamus in observational fear-related neural activity in mouse anterior cingulate cortex

AM (Anteromedial nucleus) MBA:127 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

The same ACC injection of CTB-555 also labelled a sparse population of cells in the anteromedial thalamus, indicating an AM projection to the ACC.

Fig. 1b

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

MD (Mediodorsal nucleus of thalamus) MBA:362 functionally connects to ACA (Anterior cingulate area) MBA:31 · Mouse

Halorhodopsin inhibition of MD axon terminals in the ACC, applied during the demonstrator's foot shocks, removed the shock-related calcium responses in nearly all ACC shock-responding cells imaged in observer mice.

Fig. 3a-b

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) disagrees. “The 96.4% figure is the share of shock-responding cells whose response was suppressed during terminal inhibition, not a connection probability, so the number is mislabelled.”

MD (Mediodorsal nucleus of thalamus) MBA:362 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

Injecting the retrograde tracer CTB-555 into the right ACC labelled cell bodies in the MD, showing that MD neurons send axons to the ACC.

Fig. 1a-c

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.