GABA Signaling in the Posterodorsal Medial Amygdala Mediates Stress-induced Suppression of LH Pulsatility in Female Mice

MEA GABAergic neurons nt-bqdbjsy396 projects to ARH (Arcuate hypothalamic nucleus) MBA:223 · Mouse

Cre-dependent AAV-ChR2-EYFP injected unilaterally into the MePD of Vgat-cre mice labelled GABAergic cell bodies there, and EYFP-labelled axon terminals were seen in the ipsilateral arcuate nucleus, mostly in its posterior portion, confirming an anatomical MePD GABAergic projection to the ARC.

Fig. 2J-L (and Ka, Kb, La, Lb); Results, validation section

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MEA GABAergic neurons nt-bqdbjsy396 functionally connects to ARH (Arcuate hypothalamic nucleus) MBA:223 · Mouse

Blue-light stimulation through a fibre in the ARC of ChR2-expressing MePD GABAergic terminals lengthened the LH interpulse interval in a frequency-dependent way: 10 Hz raised it from about 23 to 38 min and 20 Hz from about 23 to 62 min, whereas 2 Hz, 5 Hz and no stimulation had no effect, and light in wild-type mice without ChR2 had no effect. This indicates that activating this GABAergic input to the ARC suppresses the GnRH pulse generator output.

Fig. 5A-F; Results, final section

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