Afferent and efferent projections of the rostral anterior cingulate cortex in young and middle-aged mice

CL (Central lateral nucleus of the thalamus) MBA:575 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

CL contained sporadic retrogradely labelled cells in young males after rACC injection.

Fig. 2A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PO (Posterior complex of the thalamus) MBA:1020 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

Po was sporadically labelled by rACC retrograde tracer in young males only.

Fig. 2A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACA (Anterior cingulate area) MBA:31 synapses onto LD (Lateral dorsal nucleus of thalamus) MBA:155 · Mouse

LD showed light anterograde transsynaptic labelling from rACC in young mice, reduced in middle-aged males.

Fig. 2A, Fig. 7

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACA (Anterior cingulate area) MBA:31 synapses onto AI (Agranular insular area) MBA:95 · Mouse

Agranular insular cortex was lightly labelled by rACC anterograde transsynaptic tracing in young males.

Fig. 2A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ORB (Orbital area) MBA:714 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

Orbital cortex contained densely labelled retrograde cells ipsilaterally and lightly labelled cells contralaterally after rACC injection; it was a major input, reduced in middle-aged versus young females.

Fig. 2A, Fig. 3, Fig. 4A, Fig. 5B

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACA (Anterior cingulate area) MBA:31 synapses onto VENT (Ventral group of the dorsal thalamus) MBA:637 · Mouse

Anterograde trans-monosynaptic AAV from rACC heavily labelled VAL/VM neurons in young mice, the major output target (over 40% of output cells in most groups), with significant reduction in middle-aged mice.

Fig. 2A, Fig. 3, Fig. 4B, Fig. 6E

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) disagrees. “The 18.08% figure is the middle-aged male share, not the value for the strong connection described, where VAL/VM exceeded 40%.”

VP (Ventral posterior complex of the thalamus) MBA:709 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

No retrogradely labelled cells were found in VPL/VPM after rACC injection in any age or sex group.

Fig. 2, Fig. 3

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACA (Anterior cingulate area) MBA:31 synapses onto PCN (Paracentral nucleus) MBA:907 · Mouse

PC was moderately labelled by rACC anterograde transsynaptic virus in young males.

Fig. 2A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

IAM (Interanteromedial nucleus of the thalamus) MBA:1120 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

IAM contained only sporadic retrogradely labelled cells after rACC injection.

Fig. 2A, Fig. 3

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PCN (Paracentral nucleus) MBA:907 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

PC showed sporadic retrograde labelling after rACC injection across groups.

Fig. 2, Fig. 3

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACA (Anterior cingulate area) MBA:31 synapses onto PO (Posterior complex of the thalamus) MBA:1020 · Mouse

Po showed moderate anterograde transsynaptic labelling from rACC in young mice.

Fig. 2A, Fig. 3A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACA (Anterior cingulate area) MBA:31 synapses onto AM (Anteromedial nucleus) MBA:127 · Mouse

AM was densely labelled by the rACC anterograde transsynaptic virus in young mice, with lower counts in middle-aged mice of both sexes.

Fig. 2A, Fig. 3A, Fig. 6B

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CLA (Claustrum) MBA:583 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

The claustrum showed moderate ipsilateral retrograde labelling in young males (dense in young females), a minor input component that was lower in middle-aged females.

Fig. 2A, Fig. 3, Fig. 5A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACA (Anterior cingulate area) MBA:31 synapses onto BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

BLA neurons were moderately labelled ipsilaterally by rACC anterograde transsynaptic virus in young mice, and lightly to sporadically in middle-aged mice.

Fig. 2A, Fig. 3A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACA (Anterior cingulate area) MBA:31 synapses onto IAM (Interanteromedial nucleus of the thalamus) MBA:1120 · Mouse

IAM was lightly labelled by anterograde transsynaptic virus from rACC, with fewer cells in middle-aged males.

Fig. 2A, Fig. 6C

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

MD (Mediodorsal nucleus of thalamus) MBA:362 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

MD showed sporadic retrograde labelling from rACC in middle-aged males and young females.

Fig. 2B, Fig. 3A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACA (Anterior cingulate area) MBA:31 synapses onto LP (Lateral posterior nucleus of the thalamus) MBA:218 · Mouse

LPM was lightly labelled (moderately in young females) by rACC anterograde transsynaptic tracing.

Fig. 2A, Fig. 3A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACA (Anterior cingulate area) MBA:31 synapses onto CLA (Claustrum) MBA:583 · Mouse

The claustrum was moderately labelled by rACC anterograde transsynaptic tracing in young mice, lower in middle-aged males.

Fig. 2A, Fig. 3A, Fig. 6A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACA (Anterior cingulate area) MBA:31 synapses onto ORB (Orbital area) MBA:714 · Mouse

Orbital cortex showed moderate ipsilateral anterograde transsynaptic labelling from rACC in young mice.

Fig. 2A, Fig. 3A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACA (Anterior cingulate area) MBA:31 synapses onto CL (Central lateral nucleus of the thalamus) MBA:575 · Mouse

CL showed dense anterograde transsynaptic labelling from rACC in young mice, reduced in middle-aged males.

Fig. 2A, Fig. 3A, Fig. 6F

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACA (Anterior cingulate area) MBA:31 synapses onto PVT (Paraventricular nucleus of the thalamus) MBA:149 · Mouse

PVT contained only sporadic anterograde transsynaptic labelling from rACC in young mice.

Fig. 2A, Fig. 3A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACA (Anterior cingulate area) MBA:31 synapses onto VP (Ventral posterior complex of the thalamus) MBA:709 · Mouse

VPL/VPM showed light anterograde transsynaptic labelling from rACC in young mice.

Fig. 2A, Fig. 3A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACA (Anterior cingulate area) MBA:31 synapses onto CM (Central medial nucleus of the thalamus) MBA:599 · Mouse

CM was lightly labelled by anterograde transsynaptic virus from rACC in young mice.

Fig. 2A, Fig. 3A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

VENT (Ventral group of the dorsal thalamus) MBA:637 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

VAL/VM contained lightly labelled retrograde cells after rACC injection in young males (moderate in middle-aged males), a minor input source.

Fig. 2A, Fig. 3, Fig. 4A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CM (Central medial nucleus of the thalamus) MBA:599 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

CM was sporadically labelled by retrograde tracer from rACC in male mice.

Fig. 2

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

AI (Agranular insular area) MBA:95 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

Retrogradely labelled cells were found in the agranular insular cortex, lightly labelled in young males and moderately in other groups.

Fig. 2A, Fig. 3

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) disagrees. “AIC input was sporadic, not moderate, in middle-aged females, so the claim about the other groups is wrong.”

LD (Lateral dorsal nucleus of thalamus) MBA:155 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

LD was sporadically labelled by rACC retrograde tracer in male mice, and unlabelled in females.

Fig. 2A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ACA (Anterior cingulate area) MBA:31 synapses onto MD (Mediodorsal nucleus of thalamus) MBA:362 · Mouse

MD was densely labelled after anterograde transsynaptic tracing from rACC in young mice and decreased with age.

Fig. 2A, Fig. 3A, Fig. 6D

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

AM (Anteromedial nucleus) MBA:127 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

AM showed sporadic retrograde labelling after rACC injection (light in middle-aged males).

Fig. 2A, Fig. 3

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

LP (Lateral posterior nucleus of the thalamus) MBA:218 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

LPM showed sporadic retrograde labelling in young mice and none in middle-aged mice.

Fig. 2A, Fig. 3A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

Retrograde AAV injected into rACC labelled many ipsilateral BLA neurons, graded dense in young mice and in middle-aged males, with sporadic contralateral labelling; BLA was a major input component across all groups.

Fig. 2A, Fig. 3, Fig. 4A

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PVT (Paraventricular nucleus of the thalamus) MBA:149 projects to ACA (Anterior cingulate area) MBA:31 · Mouse

PVT contained no retrogradely labelled cells after rACC injection in any group.

Fig. 2, Fig. 3

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.