Distinct orbitofrontal circuits with dorsal and ventral CA1 differentially regulate spatial memory and emotional behaviors

ORB (Orbital area) MBA:714 projects to CA1 (Field CA1) MBA:382 · Mouse

Retrograde tracing from dorsal CA1 gave no evidence of a direct projection from the OFC.

Results, OFC–dCA1 connectivity

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CA1 (Field CA1) MBA:382 synapses onto ENT (Entorhinal area) MBA:909 · Mouse

Anterograde transsynaptic Cre from vCA1 produced tdTomato-labelled neurons in the entorhinal cortex.

Fig. 2M–P

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CA1 (Field CA1) MBA:382 projects to ORB (Orbital area) MBA:714 · Mouse

AAV2/retro-EGFP injected into the OFC of C57 mice retrogradely labelled neurons in ventral CA1, indicating a direct monosynaptic vCA1 to OFC projection.

Fig. 2Q–T

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 projects to ORB (Orbital area) MBA:714 · Mouse

BLA neurons were retrogradely labelled from the OFC, some also carrying the vCA1 transsynaptic label, consistent with a vCA1 to BLA to OFC relay.

Fig. 2D,I–L

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CLA (Claustrum) MBA:583 projects to CA1 (Field CA1) MBA:382 · Mouse

Retrograde AAV2/retro-EGFP injected into dorsal CA1 labelled claustrum neurons, some of which also carried the OFC-derived transsynaptic label, identifying the claustrum as a relay to dCA1.

Fig. 1C–G

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CA1 (Field CA1) MBA:382 synapses onto ATN (Anterior group of the dorsal thalamus) MBA:239 · Mouse

AAV2/1-Cre injected into vCA1 of Ai9 mice produced tdTomato-labelled neurons in the anterior thalamic nuclei, marking cells receiving transsynaptic input from vCA1.

Fig. 2E–H

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ATN (Anterior group of the dorsal thalamus) MBA:239 projects to ORB (Orbital area) MBA:714 · Mouse

Retrograde EGFP injected into the OFC labelled ATN neurons, some double-labelled with the vCA1 transsynaptic marker, identifying the ATN as a relay to the OFC.

Fig. 2D–H

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CA1 (Field CA1) MBA:382 synapses onto BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Anterograde transsynaptic AAV2/1-Cre from vCA1 labelled tdTomato-positive neurons in the basolateral amygdala.

Fig. 2I–L

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ENT (Entorhinal area) MBA:909 projects to ORB (Orbital area) MBA:714 · Mouse

Entorhinal neurons were retrogradely labelled by EGFP injected into the OFC, with a subset double-labelled by the vCA1 transsynaptic marker.

Fig. 2D,M–P

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ORB (Orbital area) MBA:714 synapses onto CLA (Claustrum) MBA:583 · Mouse

After AAV2/1-Cre was injected into the OFC of Ai9 mice, tdTomato-labelled neurons in the claustrum marked cells that receive anterograde transsynaptic input from the OFC.

Fig. 1C–F

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

CA1 (Field CA1) MBA:382 projects to ORB (Orbital area) MBA:714 · Mouse

Retrograde tracer injected into the OFC, combined with AAV2/1-Cre in dCA1, revealed no direct or indirect dorsal CA1 to OFC projection.

Results, OFC–dCA1 connectivity

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

ORB (Orbital area) MBA:714 projects to CA1 (Field CA1) MBA:382 · Mouse

Retrograde tracer injected into ventral CA1, combined with AAV2/1-Cre in the OFC, showed no direct or indirect OFC to vCA1 projection.

Results, OFC–vCA1 connectivity

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.