A Group of Descending Glutamatergic Neurons Activated by Stress in Corticolimbic Regions Project to the Nucleus Accumbens

HIP (Hippocampal region) MBA:1080 projects to ACB (Nucleus accumbens) MBA:56 · Mouse

Retrograde CTB from the NAc labelled ventral hippocampal neurons (about 600-680 cells/mm2), of which about 90% (883/980) were glutamate-positive; only about 1.1% were c-Fos positive after restraint.

Fig. 1G; Fig. 2G-I; Fig. 3G-I

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 projects to ACB (Nucleus accumbens) MBA:56 · Mouse

CTB injected into the NAc retrogradely labelled many BLA neurons (roughly 430-490 cells/mm2), 93% of which (610/657) were glutamate-immunopositive; about 4% of these projection cells expressed c-Fos after restraint stress.

Fig. 1F; Fig. 2D-F; Fig. 3D-F

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.

PL (Prelimbic area) MBA:972 projects to ACB (Nucleus accumbens) MBA:56 · Mouse

After bilateral injection of Alexa-conjugated CTB into the NAc lateral core and shell, retrogradely labelled cells were found in the PL (about 170 cells/mm2); roughly 90% of them (743/829) were immunopositive for glutamate, and about 2.7% were c-Fos positive after 1-h restraint.

Fig. 1E; Fig. 2A-C; Fig. 3A-C

Made by an AI model reading the paper (claude-opus-5-5, extract@0.3.0); a second AI model (claude-opus-5-5) agrees.