Longitudinal neural connection detection using a ferritin‐encoding adeno‐associated virus vector and in vivo MRI method

SCs (Superior colliculus, sensory related) MBA:302 projects to CP (Caudoputamen) MBA:672 · Mouse

The superior colliculus showed no ferritin/EGFP expression after retrograde AAV injection into the caudate putamen and served as a negative control region, with no significant MRI signal or T2 relaxation-time change at any time point.

Fig. 6; Fig. S2, S3; Results

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

AI (Agranular insular area) MBA:95 projects to CP (Caudoputamen) MBA:672 · Mouse

Following caudate putamen injection of the retrograde AAV, transgene-expressing neurons appeared in insular cortex, notably in its fifth layer, matched by localized hypointense MRI contrast and significant T2 relaxation-time changes.

Figs. 1b, 2b, 4, 6; Results

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

TH (Thalamus) MBA:549 projects to CP (Caudoputamen) MBA:672 · Mouse

Thalamic neurons expressed the retrogradely delivered ferritin transgene after caudate putamen injection, visible in fluorescence images and as significant T2 relaxation-time change in the thalamic ROI at 30 and 60 days.

Figs. 2b, 4, 5, 6; Results

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

Isocortex MBA:315 projects to CP (Caudoputamen) MBA:672 · Mouse

After injecting the retrogradely transported rAAV2-retro vector (ferritin- or EGFP-encoding) into the caudate putamen, labelled cell bodies expressing the transgene were seen in prefrontal cortex, both by fluorescence/immunostaining and as hypointense T2-weighted MRI signal and significant T2 relaxation-time changes that grew from 30 to 60 days post-injection.

Figs. 1b, 2b, 4, 5, 6; Results

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

BLA (Basolateral amygdalar nucleus) MBA:295 projects to CP (Caudoputamen) MBA:672 · Mouse

Retrograde rAAV2-retro injected into the caudate putamen labelled basolateral amygdala neurons, shown by ferritin immunofluorescence and by corresponding hypointense T2-weighted MRI signal and significant T2 relaxation-time changes by 30-60 days.

Figs. 1b, 2b, 4, 5, 6; Results

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.

HIP (Hippocampal region) MBA:1080 projects to CP (Caudoputamen) MBA:672 · Mouse

Retrograde rAAV2-retro delivered to the caudate putamen produced ferritin/EGFP-expressing neurons in the hippocampus (including a posterior hippocampal level), detected both histologically and as MRI contrast and T2 relaxation-time changes at 30 and 60 days.

Figs. 1b, 2b, 4, 5, 6; Results

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); a second AI model (claude-opus-5) agrees.