Anatomically and functionally distinct locus coeruleus efferents mediate opposing effects on anxiety-like behavior

LC (Locus ceruleus) UBERON:0002148 projects to PL (Prelimbic area) UBERON:8440032 · Rat

Fluorescent dextran injected at the border of prelimbic and infralimbic cortex retrogradely labelled LC neurons, in numbers statistically indistinguishable from those labelled from CeA, and these cells were largely distinct from the CeA-projecting population (~7.6% double-labelled). A retrogradely transported noradrenergic-selective CAV2-PRS-Cre vector injected into mPFC likewise transduced LC neurons.

Fig. 1E, 1G, 1I; Fig. 3B,D; Methods (Surgery)

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LC (Locus ceruleus) UBERON:0002148 projects to CEA (central amygdaloid nucleus) UBERON:0002883 · Rat

Fluorescent dextran injected into CeA retrogradely labelled neurons throughout the dorsoventral extent of LC, with a bias toward the dorsal part; in the same animals only about 7.6% of all labelled LC cells carried both the CeA and mPFC tracers, indicating largely separate populations. A retrogradely transported CAV2 vector with a noradrenergic-selective PRS promoter injected into CeA also transduced LC neurons, confirming the pathway arises from noradrenergic LC cells.

Fig. 1E, 1G, 1H; Fig. 3A,C; Methods (Surgery)

Made by an AI model reading the paper (claude-opus-5, extract@0.3.0); not checked yet.