Stress induces insertion of calcium-permeable AMPA receptors in the OFC–BLA synapse and modulates emotional behaviours in mice

BLA (Basolateral amygdalar nucleus) MBA:295 functionally connects to BLA glutamatergic principal neurons nt-wprma5fcrr · Mouse

Electrical stimulation within the BLA evoked mixed AMPAR/NMDAR excitatory postsynaptic currents in BLA pyramidal neurons, used as a measure of non-pathway-specific local input; the AMPA/NMDA ratio of this input was unchanged by stress.

Supplementary Fig. 1B,D,E (described in Results)

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ACA (Anterior cingulate area) MBA:31 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

AAV-driven ChR2-EYFP expression in the anterior cingulate cortex led to labelled axon terminals in the basolateral amygdala several weeks after injection.

Results, prefrontal-amygdala pathways; Supplementary Fig. 1A

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ACA (Anterior cingulate area) MBA:31 functionally connects to BLA glutamatergic principal neurons nt-wprma5fcrr · Mouse

Light stimulation of ChR2-expressing anterior cingulate axons in slices produced AMPAR- and NMDAR-mediated excitatory postsynaptic currents in BLA pyramidal neurons, establishing a functional ACC-to-BLA excitatory synapse.

Fig. 2l,m

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ORB (Orbital area) MBA:714 functionally connects to BLA glutamatergic principal neurons nt-wprma5fcrr · Mouse

Blue-light activation of ChR2-expressing orbitofrontal axons in slices evoked excitatory postsynaptic currents in BLA pyramidal neurons; the responses disappeared with tetrodotoxin and returned with 4-aminopyridine, indicating a monosynaptic glutamatergic (AMPAR/NMDAR-mediated) input.

Fig. 1c,d; Fig. 2f-k

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ORB (Orbital area) MBA:714 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

After AAV-CaMKIIa-ChR2-EYFP was injected into the lateral/ventral orbitofrontal cortex, fluorescent axon terminals from the injection site were seen in the anterior portion of the basolateral amygdala.

Fig. 1a,b; Fig. 5b

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