The primary somatosensory sensory cortex-basolateral amygdala pathway contributes to comorbid depression in spared nerve injury-induced neuropathic pain

SSp-ll (Primary somatosensory area, lower limb) MBA:337 synapses onto BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Anterograde transsynaptic AAV2/1-hSyn-Cre placed in S1HL combined with Cre-dependent AAV-DIO-EGFP in BLA labelled numerous BLA postsynaptic neurons; about 90% of the labelled BLA cells expressed CCK mRNA and about 83% expressed Slc17a7 mRNA, identifying them as CCK-positive glutamatergic neurons.

Fig. 3C1-C3, 3D, 3E

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SSp-ll (Primary somatosensory area, lower limb) MBA:337 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

A retrograde AAV (AAV2/Retro-hSyn-EGFP) injected into the BLA labelled cell bodies in S1, concentrated in the hind limb region and mainly in layer 5. RNAscope showed that roughly 90% of these retrogradely labelled cells contained Slc17a7 mRNA, indicating glutamatergic projection neurons.

Fig. 3A1-A4, 3B; Results section on circuit architecture

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