Spared nerve injury differentially alters parabrachial monosynaptic excitatory inputs to molecularly specific neurons in distinct subregions of the central amygdala

PB (Parabrachial nucleus) MBA:867 functionally connects to CEAc (Central amygdalar nucleus, capsular part) MBA:544 · Mouse

Somatostatin-negative laterocapsular central amygdala neurons received the strongest monosynaptic parabrachial input (~325 pA), significantly larger than their paired SOM-positive neighbours, with no difference in paired-pulse ratio (14 pairs).

Fig. 3D–F; Results 3.2

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PB (Parabrachial nucleus) MBA:867 functionally connects to CEAm (Central amygdalar nucleus, medial part) MBA:559 · Mouse

Eight of thirteen somatostatin-negative neurons in the medial central amygdala showed monosynaptic parabrachial EPSCs, with smaller mean amplitude (~64 pA) than paired SOM-positive cells.

Fig. 3G–I; Results 3.2

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PB (Parabrachial nucleus) MBA:867 functionally connects to CEAl somatostatin-expressing neurons nt-t6ymz01hv4 · Mouse

Optogenetic activation of parabrachial axons in TTX/4-AP elicited monosynaptic EPSCs in somatostatin-expressing lateral-division central amygdala neurons; amplitude (about 100 pA) and paired-pulse ratio (about 0.41) did not differ from neighbouring SOM-negative cells in 11 sequentially recorded pairs.

Fig. 3A–C; Results 3.2

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PB (Parabrachial nucleus) MBA:867 functionally connects to CEAl (Central amygdalar nucleus, lateral part) MBA:551 · Mouse

Somatostatin-negative neurons of the lateral division of the central amygdala also showed monosynaptic light-evoked EPSCs from parabrachial axons, with amplitudes (~127 pA) statistically indistinguishable from paired SOM-positive cells.

Fig. 3A–C; Results 3.2

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PB (Parabrachial nucleus) MBA:867 functionally connects to CEA (Central amygdalar nucleus) MBA:536 · Mouse

Wide-field blue-light stimulation of ChR2-expressing parabrachial axons in TTX plus 4-AP evoked monosynaptic EPSCs in all six central amygdala neurons tested, and these currents were abolished by the AMPA-receptor blocker NBQX; no monosynaptic IPSCs were seen.

Fig. 2C–E; Results 3.2

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PB (Parabrachial nucleus) MBA:867 projects to CEA (Central amygdalar nucleus) MBA:536 · Mouse

AAV expressing ChR2-Venus injected into the right lateral parabrachial nucleus produced YFP-labelled axons distributed across all central amygdala subdivisions in both SOM-tdTomato and CRH-tdTomato reporter mice.

Fig. 1H, 1I, 1Q, 1R; Results 3.1

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PB (Parabrachial nucleus) MBA:867 functionally connects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Photostimulation of parabrachial ChR2-positive axons evoked neither monosynaptic EPSCs nor IPSCs in basolateral amygdala neurons recorded as a negative control (7 BLA cells).

Fig. 2A–B; Results 3.2

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PB (Parabrachial nucleus) MBA:867 functionally connects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

Light activation of parabrachial axons produced no monosynaptic excitatory or inhibitory currents in the two lateral amygdala neurons recorded.

Fig. 2A–B; Results 3.2

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PB (Parabrachial nucleus) MBA:867 projects to BLA (Basolateral amygdalar nucleus) MBA:295 · Mouse

Labelled parabrachial axons were not detected in the basolateral amygdala, in contrast to the dense labelling in the central amygdala.

Fig. 1H; Results 3.1

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PB (Parabrachial nucleus) MBA:867 projects to LA (Lateral amygdalar nucleus) MBA:131 · Mouse

No YFP-labelled parabrachial axons were seen in the lateral amygdala after ChR2-Venus AAV injection in the lateral parabrachial nucleus.

Fig. 1H; Results 3.1

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PB (Parabrachial nucleus) MBA:867 projects to CEAc (Central amygdalar nucleus, capsular part) MBA:544 · Mouse

Within the central amygdala, the labelled parabrachial axon terminals were densest in the laterocapsular subdivision.

Fig. 1H–I; Results 3.1

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PB (Parabrachial nucleus) MBA:867 functionally connects to CEAm (Central amygdalar nucleus, medial part) MBA:559 · Mouse

Five of eight CRH-negative medial central amygdala neurons showed monosynaptic parabrachial EPSCs upon optogenetic stimulation.

Fig. 4D–F; Results 3.2

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PB (Parabrachial nucleus) MBA:867 functionally connects to CEAl (Central amygdalar nucleus, lateral part) MBA:551 · Mouse

CRH-negative lateral central amygdala neurons likewise received monosynaptic parabrachial excitatory input (~92 pA), with amplitude and paired-pulse ratio similar to paired CRH-positive cells.

Fig. 4A–C; Results 3.2

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